Improvement of Neurological Function in Rats with Ischemic Stroke by Adipose-derived Pericytes

Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovas...

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Published in:Cell transplantation 2020-01, Vol.29, p.963689720956956-963689720956956
Main Authors: Ogay, Vyacheslav, Kumasheva, Venera, Li, Yelena, Mukhlis, Sholpan, Sekenova, Aliya, Olzhayev, Farkhad, Tsoy, Andrey, Umbayev, Baurzhan, Askarova, Sholpan, Shpekov, Azat, Kaliyev, Assylbek, Zhetpisbayev, Berik, Makhambetov, Yerbol, Akshulakov, Serik, Saparov, Arman, Ramankulov, Yerlan
Format: Article
Language:English
Subjects:
Adipose tissue
Angiogenesis
Capillaries
Cerebral blood flow
Doublecortin protein
Flow cytometry
Functional plasticity
Immunohistochemistry
Injection
Intravenous administration
Ischemia
Neostriatum
Neuroblasts
Neurogenesis
Neurotrophic factors
Original
Pericytes
Recovery of function
Rodents
Stroke
Transplantation
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Summary:Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were isolated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neurogenesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline–treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.
ISSN:0963-6897
1555-3892
DOI:10.1177/0963689720956956