Screening and characterisation of bacteriocin-producing bacteria capable of inhibiting the growth of bovine mastitis

A total of 302 bacterial strains were isolated from 43 raw milk samples collected from dairy farms in Chiang Mai province. Out of these strains isolated, three strains were found to produce bacteriocins against Streptococcus dysgalactiae DMST10953, a bovine mastitis pathogen. These were Lactobacillu...

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Bibliographic Details
Published in:Maejo international journal of science and technology 2009-01, Vol.3 (1), p.43-52
Main Author: Piyanuch Niamsup
Format: Article
Language:English
Subjects:
bacteriocin
bovine mastitis
Streptococcus dysgalactiae
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Summary:A total of 302 bacterial strains were isolated from 43 raw milk samples collected from dairy farms in Chiang Mai province. Out of these strains isolated, three strains were found to produce bacteriocins against Streptococcus dysgalactiae DMST10953, a bovine mastitis pathogen. These were Lactobacillus fermentum RMM701, Streptococcus bovis RMM703 and Streptococcus bovis RMM902, which exhibited bacteriocin activity at 40, 40 and 20 AU/ml respectively. Bacteriocins produced by these three strains were heat-stable up to 80 oC. Bacteriocins produced by L. fermentum RMM701 and S. bovis RMM902 were stable at pH 2.0 - 7.0 while that from S. bovis RMM703 was stable at pH 2.0 - 6.0. These bacteriocins were also found to be inactivated by proteolytic enzymes such as proteinase K and subtilisin A.
ISSN:1905-7873
1905-7873