Metagenomic versus targeted next-generation sequencing for detection of microorganisms in bronchoalveolar lavage fluid among renal transplantation recipients

Metagenomic next-generation sequencing (mNGS), which provides untargeted and unbiased pathogens detection, has been extensively applied to improve diagnosis of pulmonary infection. This study aimed to compare the clinical performance between mNGS and targeted NGS (tNGS) for microbial detection and i...

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Published in:Frontiers in immunology 2024-08, Vol.15, p.1443057
Main Authors: Huang, Zhaoru, Hu, Bingxue, Li, Jinfeng, Feng, Min, Wang, Zhigang, Huang, Fengxiang, Xu, Huan, Liu, Lei, Shang, Wenjun
Format: Article
Language:English
Subjects:
Adult
Aged
Bacteria - genetics
Bacteria - isolation & purification
Bronchoalveolar Lavage Fluid - microbiology
Female
Fungi - genetics
Fungi - isolation & purification
High-Throughput Nucleotide Sequencing - methods
Humans
Immunology
kidney transplantation
Kidney Transplantation - adverse effects
Male
Metagenomics - methods
metagenomics Next-Generation Sequencing (mNGS)
Middle Aged
pulmonary infection
respiratory pathogens
targeted next-generation sequencing (tNGS)
Torque teno virus
Transplant Recipients
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Summary:Metagenomic next-generation sequencing (mNGS), which provides untargeted and unbiased pathogens detection, has been extensively applied to improve diagnosis of pulmonary infection. This study aimed to compare the clinical performance between mNGS and targeted NGS (tNGS) for microbial detection and identification in bronchoalveolar lavage fluid (BALF) from kidney transplantation recipients (KTRs). BALF samples with microbiological results from mNGS and conventional microbiological test (CMT) were included. For tNGS, samples were extracted, amplified by polymerase chain reaction with pathogen-specific primers, and sequenced on an Illumina Nextseq. A total of 99 BALF from 99 KTRs, among which 93 were diagnosed as pulmonary infection, were analyzed. Compared with CMT, both mNGS and tNGS showed higher positive rate and sensitivity (p
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2024.1443057