Oral human cytomegalovirus prevalence and its relationships with periodontitis and Porphyromonas gingivalis in Japanese adults: a cross-sectional study

This study aimed to clarify the association between oral human cytomegalovirus (HCMV) and periodontitis in Japanese adults. In total, 190 patients (75 men and 115 women; mean age, 70.2 years) who visited Hiroshima University Hospital between March 2018 and May 2020 were included. Oral rinse samples...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied oral science 2020-01, Vol.28, p.e20200501-e20200501
Main Authors: Nakamura, Mariko, Shigeishi, Hideo, Cheng-Yih, S U, Sugiyama, Masaru, Ohta, Kouji
Format: Article
Language:English
Subjects:
Aged
Bacteroidaceae Infections - epidemiology
Coinfection
Cross-Sectional Studies
Cytomegalovirus
Cytomegalovirus Infections - epidemiology
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Female
Human Cytomegalovirus
Humans
Japan - epidemiology
Male
Original
Periodontal Pocket - microbiology
Periodontal Pocket - virology
Periodontitis
Periodontitis - epidemiology
Periodontitis - microbiology
Periodontitis - virology
Porphyromonas gingivalis
Prevalence
Real-time PCR
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c513t-895d4c18980491a7258c17a264e8d3828f8c590f5c9926855802d611540101d43
cites cdi_FETCH-LOGICAL-c513t-895d4c18980491a7258c17a264e8d3828f8c590f5c9926855802d611540101d43
container_end_page e20200501
container_issue
container_start_page e20200501
container_title Journal of applied oral science
container_volume 28
creator Nakamura, Mariko
Shigeishi, Hideo
Cheng-Yih, S U
Sugiyama, Masaru
Ohta, Kouji
description This study aimed to clarify the association between oral human cytomegalovirus (HCMV) and periodontitis in Japanese adults. In total, 190 patients (75 men and 115 women; mean age, 70.2 years) who visited Hiroshima University Hospital between March 2018 and May 2020 were included. Oral rinse samples were taken to examine the presence of HCMV DNA using real-time polymerase chain reaction (PCR). P. gingivalis was detected by semi-quantitative PCR analysis. HCMV DNA was present in nine of 190 patients (4.7%). There were significant associations between HCMV presence and the presence of ≥4-mm-deep periodontal pockets with bleeding on probing (BOP) (P
doi_str_mv 10.1590/1678-7757-2020-00501
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_fff29f2d159d40199cee7c90b3c37ffb</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S1678_77572020000100487</scielo_id><doaj_id>oai_doaj_org_article_fff29f2d159d40199cee7c90b3c37ffb</doaj_id><sourcerecordid>2470901268</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-895d4c18980491a7258c17a264e8d3828f8c590f5c9926855802d611540101d43</originalsourceid><addsrcrecordid>eNpVkl2P1CAUhhujcdfVf2AMl9505aMU8MLEbPxYs8maqNeEAdoyaaECnc38Ev-udGacuNxADud9eDnnVNVrBK8RFfAdahmvGaOsxhDDGkIK0ZPq8hRu6dPzmbKL6kVKWwgJJkw8ry5IWYgIdFn9uY9qBMMyKQ_0PofJ9moMOxeXBOZod2q0XlugvAEuJxDtqLILPg1uTuDB5QHMNrpggs8uu3RI_B7iPOxjmIJXCfTO965wyqXz4JualbepEM0y5vQeKKBjSKlOVq_gYiblxexfVs86NSb76rRfVb8-f_p587W-u_9ye_PxrtYUkVxzQU2jERccNgIphinXiCncNpYbwjHvuC7F6qgWArecUg6xaRGiDUQQmYZcVbdHrglqK-foJhX3MignD4EQe6lidnq0sus6LDpsSvVNkQuhrWVawA3RhHXdprCuj6yknR2D3IYllg8l-WNthFwbsbaqLARhw1kRfDgK5mUzWaOtz6Ubj1w8vvFukH3YFZQglMACeHsCxPB7sSnLySVtx7HUOCxJ4oZBAVH5eUltjqmHckfbnZ9BUK4DJc8m5epSHgaqyN78b_Es-jdB5C-CA8ex</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2470901268</pqid></control><display><type>article</type><title>Oral human cytomegalovirus prevalence and its relationships with periodontitis and Porphyromonas gingivalis in Japanese adults: a cross-sectional study</title><source>PubMed (Medline)</source><source>SciELO</source><creator>Nakamura, Mariko ; Shigeishi, Hideo ; Cheng-Yih, S U ; Sugiyama, Masaru ; Ohta, Kouji</creator><creatorcontrib>Nakamura, Mariko ; Shigeishi, Hideo ; Cheng-Yih, S U ; Sugiyama, Masaru ; Ohta, Kouji</creatorcontrib><description>This study aimed to clarify the association between oral human cytomegalovirus (HCMV) and periodontitis in Japanese adults. In total, 190 patients (75 men and 115 women; mean age, 70.2 years) who visited Hiroshima University Hospital between March 2018 and May 2020 were included. Oral rinse samples were taken to examine the presence of HCMV DNA using real-time polymerase chain reaction (PCR). P. gingivalis was detected by semi-quantitative PCR analysis. HCMV DNA was present in nine of 190 patients (4.7%). There were significant associations between HCMV presence and the presence of ≥4-mm-deep periodontal pockets with bleeding on probing (BOP) (P&lt;0.01) and ≥6-mm-deep periodontal pockets with BOP (P=0.01). However, no significant relationship was observed between HCMV presence and periodontal epithelial surface area scores. Logistic regression analysis revealed that the presence of ≥4-mm-deep periodontal pockets with BOP was significantly associated with HCMV (odds ratio, 14.4; P=0.01). Propensity score matching was performed between patients presenting ≥4-mm-deep periodontal pockets with BOP (i.e., active periodontitis) and patients without ≥4-mm-deep periodontal pockets with BOP; 62 matched pairs were generated. Patients who had ≥4-mm-deep periodontal pockets with BOP showed a higher rate of HCMV presence (9.7%) than those who lacked ≥4-mm-deep periodontal pockets with BOP (0.0%). There was a significant relationship between HCMV presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). A significant relationship was found between HCMV/P. gingivalis DNA presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). Coinfection of oral HCMV and P. gingivalis was significantly associated with active periodontitis. Moreover, interactions between oral HCMV and P. gingivalis may be related to the severity of periodontal disease.</description><identifier>ISSN: 1678-7757</identifier><identifier>ISSN: 1678-7765</identifier><identifier>EISSN: 1678-7765</identifier><identifier>DOI: 10.1590/1678-7757-2020-00501</identifier><identifier>PMID: 33331391</identifier><language>eng</language><publisher>Brazil: Faculdade De Odontologia De Bauru - USP</publisher><subject>Aged ; Bacteroidaceae Infections - epidemiology ; Coinfection ; Cross-Sectional Studies ; Cytomegalovirus ; Cytomegalovirus Infections - epidemiology ; Dentistry ; DENTISTRY, ORAL SURGERY &amp; MEDICINE ; Female ; Human Cytomegalovirus ; Humans ; Japan - epidemiology ; Male ; Original ; Periodontal Pocket - microbiology ; Periodontal Pocket - virology ; Periodontitis ; Periodontitis - epidemiology ; Periodontitis - microbiology ; Periodontitis - virology ; Porphyromonas gingivalis ; Prevalence ; Real-time PCR</subject><ispartof>Journal of applied oral science, 2020-01, Vol.28, p.e20200501-e20200501</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-895d4c18980491a7258c17a264e8d3828f8c590f5c9926855802d611540101d43</citedby><cites>FETCH-LOGICAL-c513t-895d4c18980491a7258c17a264e8d3828f8c590f5c9926855802d611540101d43</cites><orcidid>0000-0001-6593-956X ; 0000-0003-0883-4299 ; 0000-0002-0658-1358 ; 0000-0003-3844-1517 ; 0000-0001-9782-5495</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793530/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793530/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,24130,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33331391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakamura, Mariko</creatorcontrib><creatorcontrib>Shigeishi, Hideo</creatorcontrib><creatorcontrib>Cheng-Yih, S U</creatorcontrib><creatorcontrib>Sugiyama, Masaru</creatorcontrib><creatorcontrib>Ohta, Kouji</creatorcontrib><title>Oral human cytomegalovirus prevalence and its relationships with periodontitis and Porphyromonas gingivalis in Japanese adults: a cross-sectional study</title><title>Journal of applied oral science</title><addtitle>J Appl Oral Sci</addtitle><description>This study aimed to clarify the association between oral human cytomegalovirus (HCMV) and periodontitis in Japanese adults. In total, 190 patients (75 men and 115 women; mean age, 70.2 years) who visited Hiroshima University Hospital between March 2018 and May 2020 were included. Oral rinse samples were taken to examine the presence of HCMV DNA using real-time polymerase chain reaction (PCR). P. gingivalis was detected by semi-quantitative PCR analysis. HCMV DNA was present in nine of 190 patients (4.7%). There were significant associations between HCMV presence and the presence of ≥4-mm-deep periodontal pockets with bleeding on probing (BOP) (P&lt;0.01) and ≥6-mm-deep periodontal pockets with BOP (P=0.01). However, no significant relationship was observed between HCMV presence and periodontal epithelial surface area scores. Logistic regression analysis revealed that the presence of ≥4-mm-deep periodontal pockets with BOP was significantly associated with HCMV (odds ratio, 14.4; P=0.01). Propensity score matching was performed between patients presenting ≥4-mm-deep periodontal pockets with BOP (i.e., active periodontitis) and patients without ≥4-mm-deep periodontal pockets with BOP; 62 matched pairs were generated. Patients who had ≥4-mm-deep periodontal pockets with BOP showed a higher rate of HCMV presence (9.7%) than those who lacked ≥4-mm-deep periodontal pockets with BOP (0.0%). There was a significant relationship between HCMV presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). A significant relationship was found between HCMV/P. gingivalis DNA presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). Coinfection of oral HCMV and P. gingivalis was significantly associated with active periodontitis. Moreover, interactions between oral HCMV and P. gingivalis may be related to the severity of periodontal disease.</description><subject>Aged</subject><subject>Bacteroidaceae Infections - epidemiology</subject><subject>Coinfection</subject><subject>Cross-Sectional Studies</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus Infections - epidemiology</subject><subject>Dentistry</subject><subject>DENTISTRY, ORAL SURGERY &amp; MEDICINE</subject><subject>Female</subject><subject>Human Cytomegalovirus</subject><subject>Humans</subject><subject>Japan - epidemiology</subject><subject>Male</subject><subject>Original</subject><subject>Periodontal Pocket - microbiology</subject><subject>Periodontal Pocket - virology</subject><subject>Periodontitis</subject><subject>Periodontitis - epidemiology</subject><subject>Periodontitis - microbiology</subject><subject>Periodontitis - virology</subject><subject>Porphyromonas gingivalis</subject><subject>Prevalence</subject><subject>Real-time PCR</subject><issn>1678-7757</issn><issn>1678-7765</issn><issn>1678-7765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkl2P1CAUhhujcdfVf2AMl9505aMU8MLEbPxYs8maqNeEAdoyaaECnc38Ev-udGacuNxADud9eDnnVNVrBK8RFfAdahmvGaOsxhDDGkIK0ZPq8hRu6dPzmbKL6kVKWwgJJkw8ry5IWYgIdFn9uY9qBMMyKQ_0PofJ9moMOxeXBOZod2q0XlugvAEuJxDtqLILPg1uTuDB5QHMNrpggs8uu3RI_B7iPOxjmIJXCfTO965wyqXz4JualbepEM0y5vQeKKBjSKlOVq_gYiblxexfVs86NSb76rRfVb8-f_p587W-u_9ye_PxrtYUkVxzQU2jERccNgIphinXiCncNpYbwjHvuC7F6qgWArecUg6xaRGiDUQQmYZcVbdHrglqK-foJhX3MignD4EQe6lidnq0sus6LDpsSvVNkQuhrWVawA3RhHXdprCuj6yknR2D3IYllg8l-WNthFwbsbaqLARhw1kRfDgK5mUzWaOtz6Ubj1w8vvFukH3YFZQglMACeHsCxPB7sSnLySVtx7HUOCxJ4oZBAVH5eUltjqmHckfbnZ9BUK4DJc8m5epSHgaqyN78b_Es-jdB5C-CA8ex</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Nakamura, Mariko</creator><creator>Shigeishi, Hideo</creator><creator>Cheng-Yih, S U</creator><creator>Sugiyama, Masaru</creator><creator>Ohta, Kouji</creator><general>Faculdade De Odontologia De Bauru - USP</general><general>University of São Paulo</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>GPN</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6593-956X</orcidid><orcidid>https://orcid.org/0000-0003-0883-4299</orcidid><orcidid>https://orcid.org/0000-0002-0658-1358</orcidid><orcidid>https://orcid.org/0000-0003-3844-1517</orcidid><orcidid>https://orcid.org/0000-0001-9782-5495</orcidid></search><sort><creationdate>20200101</creationdate><title>Oral human cytomegalovirus prevalence and its relationships with periodontitis and Porphyromonas gingivalis in Japanese adults: a cross-sectional study</title><author>Nakamura, Mariko ; Shigeishi, Hideo ; Cheng-Yih, S U ; Sugiyama, Masaru ; Ohta, Kouji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-895d4c18980491a7258c17a264e8d3828f8c590f5c9926855802d611540101d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aged</topic><topic>Bacteroidaceae Infections - epidemiology</topic><topic>Coinfection</topic><topic>Cross-Sectional Studies</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus Infections - epidemiology</topic><topic>Dentistry</topic><topic>DENTISTRY, ORAL SURGERY &amp; MEDICINE</topic><topic>Female</topic><topic>Human Cytomegalovirus</topic><topic>Humans</topic><topic>Japan - epidemiology</topic><topic>Male</topic><topic>Original</topic><topic>Periodontal Pocket - microbiology</topic><topic>Periodontal Pocket - virology</topic><topic>Periodontitis</topic><topic>Periodontitis - epidemiology</topic><topic>Periodontitis - microbiology</topic><topic>Periodontitis - virology</topic><topic>Porphyromonas gingivalis</topic><topic>Prevalence</topic><topic>Real-time PCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamura, Mariko</creatorcontrib><creatorcontrib>Shigeishi, Hideo</creatorcontrib><creatorcontrib>Cheng-Yih, S U</creatorcontrib><creatorcontrib>Sugiyama, Masaru</creatorcontrib><creatorcontrib>Ohta, Kouji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of applied oral science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura, Mariko</au><au>Shigeishi, Hideo</au><au>Cheng-Yih, S U</au><au>Sugiyama, Masaru</au><au>Ohta, Kouji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oral human cytomegalovirus prevalence and its relationships with periodontitis and Porphyromonas gingivalis in Japanese adults: a cross-sectional study</atitle><jtitle>Journal of applied oral science</jtitle><addtitle>J Appl Oral Sci</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>28</volume><spage>e20200501</spage><epage>e20200501</epage><pages>e20200501-e20200501</pages><issn>1678-7757</issn><issn>1678-7765</issn><eissn>1678-7765</eissn><abstract>This study aimed to clarify the association between oral human cytomegalovirus (HCMV) and periodontitis in Japanese adults. In total, 190 patients (75 men and 115 women; mean age, 70.2 years) who visited Hiroshima University Hospital between March 2018 and May 2020 were included. Oral rinse samples were taken to examine the presence of HCMV DNA using real-time polymerase chain reaction (PCR). P. gingivalis was detected by semi-quantitative PCR analysis. HCMV DNA was present in nine of 190 patients (4.7%). There were significant associations between HCMV presence and the presence of ≥4-mm-deep periodontal pockets with bleeding on probing (BOP) (P&lt;0.01) and ≥6-mm-deep periodontal pockets with BOP (P=0.01). However, no significant relationship was observed between HCMV presence and periodontal epithelial surface area scores. Logistic regression analysis revealed that the presence of ≥4-mm-deep periodontal pockets with BOP was significantly associated with HCMV (odds ratio, 14.4; P=0.01). Propensity score matching was performed between patients presenting ≥4-mm-deep periodontal pockets with BOP (i.e., active periodontitis) and patients without ≥4-mm-deep periodontal pockets with BOP; 62 matched pairs were generated. Patients who had ≥4-mm-deep periodontal pockets with BOP showed a higher rate of HCMV presence (9.7%) than those who lacked ≥4-mm-deep periodontal pockets with BOP (0.0%). There was a significant relationship between HCMV presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). A significant relationship was found between HCMV/P. gingivalis DNA presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). Coinfection of oral HCMV and P. gingivalis was significantly associated with active periodontitis. Moreover, interactions between oral HCMV and P. gingivalis may be related to the severity of periodontal disease.</abstract><cop>Brazil</cop><pub>Faculdade De Odontologia De Bauru - USP</pub><pmid>33331391</pmid><doi>10.1590/1678-7757-2020-00501</doi><orcidid>https://orcid.org/0000-0001-6593-956X</orcidid><orcidid>https://orcid.org/0000-0003-0883-4299</orcidid><orcidid>https://orcid.org/0000-0002-0658-1358</orcidid><orcidid>https://orcid.org/0000-0003-3844-1517</orcidid><orcidid>https://orcid.org/0000-0001-9782-5495</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1678-7757
ispartof Journal of applied oral science, 2020-01, Vol.28, p.e20200501-e20200501
issn 1678-7757
1678-7765
1678-7765
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_fff29f2d159d40199cee7c90b3c37ffb
source PubMed (Medline); SciELO
subjects Aged
Bacteroidaceae Infections - epidemiology
Coinfection
Cross-Sectional Studies
Cytomegalovirus
Cytomegalovirus Infections - epidemiology
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Female
Human Cytomegalovirus
Humans
Japan - epidemiology
Male
Original
Periodontal Pocket - microbiology
Periodontal Pocket - virology
Periodontitis
Periodontitis - epidemiology
Periodontitis - microbiology
Periodontitis - virology
Porphyromonas gingivalis
Prevalence
Real-time PCR
title Oral human cytomegalovirus prevalence and its relationships with periodontitis and Porphyromonas gingivalis in Japanese adults: a cross-sectional study
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T23%3A01%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Oral%20human%20cytomegalovirus%20prevalence%20and%20its%20relationships%20with%20periodontitis%20and%20Porphyromonas%20gingivalis%20in%20Japanese%20adults:%20a%20cross-sectional%20study&rft.jtitle=Journal%20of%20applied%20oral%20science&rft.au=Nakamura,%20Mariko&rft.date=2020-01-01&rft.volume=28&rft.spage=e20200501&rft.epage=e20200501&rft.pages=e20200501-e20200501&rft.issn=1678-7757&rft.eissn=1678-7765&rft_id=info:doi/10.1590/1678-7757-2020-00501&rft_dat=%3Cproquest_doaj_%3E2470901268%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c513t-895d4c18980491a7258c17a264e8d3828f8c590f5c9926855802d611540101d43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2470901268&rft_id=info:pmid/33331391&rft_scielo_id=S1678_77572020000100487&rfr_iscdi=true