FLUORESCENT ANTIBODY METHODS AS A HELP IN THE CLINICAL DIAGNOSIS OF STREPTOCOCCAL SORE THROAT

Duplicate throat swabs were collected from patients with suspected clinial picture of streptococcal sore throat. A sheep blood agar plate was prepared and the swabs immersed in Bacto Todd-Hewitt Broth and incubated for at least a period of two hours at 37 C. After 24 hours incubation at 37 C typical...

Full description

Saved in:
Bibliographic Details
Main Authors: SHUEY,H E, ESTELA,L A
Format: Report
Language:English
Subjects:
ANTIBIOTICS
BACTERIA
BIOLOGICAL STAINS
BIOLOGY
DYES
MEDICAL EXAMINATION
MOUTH
STREPTOCOCCUS
TEST METHODS
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue
container_start_page
container_title
container_volume
creator SHUEY,H E
ESTELA,L A
description Duplicate throat swabs were collected from patients with suspected clinial picture of streptococcal sore throat. A sheep blood agar plate was prepared and the swabs immersed in Bacto Todd-Hewitt Broth and incubated for at least a period of two hours at 37 C. After 24 hours incubation at 37 C typical beta hemolytic streptococcus colonies were picked from the plates and used for the precipitin and bacitracin discs (2 units) sensitivity tests. Two smears were made from homogenates on thoroughly cleaned slide . The smears were allowed to air dry, fixed in absolute alcohol for one minute and stained with fluorescein tagged group A streptococcus antiserum for 30 minutes. After staining, the smears were rinsed in buffered saline, blotted dry and mounted with buffered glycerol saline. These smears were then observed for pres nce or absence of characteristic yellow fluorescent streptococci with ultraviolet light from a specially fitted dark field microscope. Results obtained this way were correlated with those obtained by the precipitin technique and the bacitracin disc inhibition. (Author)
format report
fullrecord <record><control><sourceid>dtic_1RU</sourceid><recordid>TN_cdi_dtic_stinet_AD0259859</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>AD0259859</sourcerecordid><originalsourceid>FETCH-dtic_stinet_AD02598593</originalsourceid><addsrcrecordid>eNrjZIh18wn1D3INdnb1C1Fw9AvxdPJ3iVTwdQ3x8HcJVnAEIgUPV58ABU8_hRAPVwVnH08_T2dHHwUXT0d3P_9gz2AFfzeF4JAg14AQf2d_Z5BUMNA8oOIgf8cQHgbWtMSc4lReKM3NIOPmGuLsoZtSkpkcX1ySmZdaEu_oYmBkamlhamlMQBoA2f4vjg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>report</recordtype></control><display><type>report</type><title>FLUORESCENT ANTIBODY METHODS AS A HELP IN THE CLINICAL DIAGNOSIS OF STREPTOCOCCAL SORE THROAT</title><source>DTIC Technical Reports</source><creator>SHUEY,H E ; ESTELA,L A</creator><creatorcontrib>SHUEY,H E ; ESTELA,L A ; FITZSIMONS GENERAL HOSPITAL DENVER COLO</creatorcontrib><description>Duplicate throat swabs were collected from patients with suspected clinial picture of streptococcal sore throat. A sheep blood agar plate was prepared and the swabs immersed in Bacto Todd-Hewitt Broth and incubated for at least a period of two hours at 37 C. After 24 hours incubation at 37 C typical beta hemolytic streptococcus colonies were picked from the plates and used for the precipitin and bacitracin discs (2 units) sensitivity tests. Two smears were made from homogenates on thoroughly cleaned slide . The smears were allowed to air dry, fixed in absolute alcohol for one minute and stained with fluorescein tagged group A streptococcus antiserum for 30 minutes. After staining, the smears were rinsed in buffered saline, blotted dry and mounted with buffered glycerol saline. These smears were then observed for pres nce or absence of characteristic yellow fluorescent streptococci with ultraviolet light from a specially fitted dark field microscope. Results obtained this way were correlated with those obtained by the precipitin technique and the bacitracin disc inhibition. (Author)</description><language>eng</language><subject>ANTIBIOTICS ; BACTERIA ; BIOLOGICAL STAINS ; BIOLOGY ; DYES ; MEDICAL EXAMINATION ; MOUTH ; STREPTOCOCCUS ; TEST METHODS</subject><creationdate>1961</creationdate><rights>APPROVED FOR PUBLIC RELEASE</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,776,881,27544,27545</link.rule.ids><linktorsrc>$$Uhttps://apps.dtic.mil/sti/citations/AD0259859$$EView_record_in_DTIC$$FView_record_in_$$GDTIC$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHUEY,H E</creatorcontrib><creatorcontrib>ESTELA,L A</creatorcontrib><creatorcontrib>FITZSIMONS GENERAL HOSPITAL DENVER COLO</creatorcontrib><title>FLUORESCENT ANTIBODY METHODS AS A HELP IN THE CLINICAL DIAGNOSIS OF STREPTOCOCCAL SORE THROAT</title><description>Duplicate throat swabs were collected from patients with suspected clinial picture of streptococcal sore throat. A sheep blood agar plate was prepared and the swabs immersed in Bacto Todd-Hewitt Broth and incubated for at least a period of two hours at 37 C. After 24 hours incubation at 37 C typical beta hemolytic streptococcus colonies were picked from the plates and used for the precipitin and bacitracin discs (2 units) sensitivity tests. Two smears were made from homogenates on thoroughly cleaned slide . The smears were allowed to air dry, fixed in absolute alcohol for one minute and stained with fluorescein tagged group A streptococcus antiserum for 30 minutes. After staining, the smears were rinsed in buffered saline, blotted dry and mounted with buffered glycerol saline. These smears were then observed for pres nce or absence of characteristic yellow fluorescent streptococci with ultraviolet light from a specially fitted dark field microscope. Results obtained this way were correlated with those obtained by the precipitin technique and the bacitracin disc inhibition. (Author)</description><subject>ANTIBIOTICS</subject><subject>BACTERIA</subject><subject>BIOLOGICAL STAINS</subject><subject>BIOLOGY</subject><subject>DYES</subject><subject>MEDICAL EXAMINATION</subject><subject>MOUTH</subject><subject>STREPTOCOCCUS</subject><subject>TEST METHODS</subject><fulltext>true</fulltext><rsrctype>report</rsrctype><creationdate>1961</creationdate><recordtype>report</recordtype><sourceid>1RU</sourceid><recordid>eNrjZIh18wn1D3INdnb1C1Fw9AvxdPJ3iVTwdQ3x8HcJVnAEIgUPV58ABU8_hRAPVwVnH08_T2dHHwUXT0d3P_9gz2AFfzeF4JAg14AQf2d_Z5BUMNA8oOIgf8cQHgbWtMSc4lReKM3NIOPmGuLsoZtSkpkcX1ySmZdaEu_oYmBkamlhamlMQBoA2f4vjg</recordid><startdate>19610630</startdate><enddate>19610630</enddate><creator>SHUEY,H E</creator><creator>ESTELA,L A</creator><scope>1RU</scope><scope>BHM</scope></search><sort><creationdate>19610630</creationdate><title>FLUORESCENT ANTIBODY METHODS AS A HELP IN THE CLINICAL DIAGNOSIS OF STREPTOCOCCAL SORE THROAT</title><author>SHUEY,H E ; ESTELA,L A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-dtic_stinet_AD02598593</frbrgroupid><rsrctype>reports</rsrctype><prefilter>reports</prefilter><language>eng</language><creationdate>1961</creationdate><topic>ANTIBIOTICS</topic><topic>BACTERIA</topic><topic>BIOLOGICAL STAINS</topic><topic>BIOLOGY</topic><topic>DYES</topic><topic>MEDICAL EXAMINATION</topic><topic>MOUTH</topic><topic>STREPTOCOCCUS</topic><topic>TEST METHODS</topic><toplevel>online_resources</toplevel><creatorcontrib>SHUEY,H E</creatorcontrib><creatorcontrib>ESTELA,L A</creatorcontrib><creatorcontrib>FITZSIMONS GENERAL HOSPITAL DENVER COLO</creatorcontrib><collection>DTIC Technical Reports</collection><collection>DTIC STINET</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SHUEY,H E</au><au>ESTELA,L A</au><aucorp>FITZSIMONS GENERAL HOSPITAL DENVER COLO</aucorp><format>book</format><genre>unknown</genre><ristype>RPRT</ristype><btitle>FLUORESCENT ANTIBODY METHODS AS A HELP IN THE CLINICAL DIAGNOSIS OF STREPTOCOCCAL SORE THROAT</btitle><date>1961-06-30</date><risdate>1961</risdate><abstract>Duplicate throat swabs were collected from patients with suspected clinial picture of streptococcal sore throat. A sheep blood agar plate was prepared and the swabs immersed in Bacto Todd-Hewitt Broth and incubated for at least a period of two hours at 37 C. After 24 hours incubation at 37 C typical beta hemolytic streptococcus colonies were picked from the plates and used for the precipitin and bacitracin discs (2 units) sensitivity tests. Two smears were made from homogenates on thoroughly cleaned slide . The smears were allowed to air dry, fixed in absolute alcohol for one minute and stained with fluorescein tagged group A streptococcus antiserum for 30 minutes. After staining, the smears were rinsed in buffered saline, blotted dry and mounted with buffered glycerol saline. These smears were then observed for pres nce or absence of characteristic yellow fluorescent streptococci with ultraviolet light from a specially fitted dark field microscope. Results obtained this way were correlated with those obtained by the precipitin technique and the bacitracin disc inhibition. (Author)</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_dtic_stinet_AD0259859
source DTIC Technical Reports
subjects ANTIBIOTICS
BACTERIA
BIOLOGICAL STAINS
BIOLOGY
DYES
MEDICAL EXAMINATION
MOUTH
STREPTOCOCCUS
TEST METHODS
title FLUORESCENT ANTIBODY METHODS AS A HELP IN THE CLINICAL DIAGNOSIS OF STREPTOCOCCAL SORE THROAT
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T02%3A50%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-dtic_1RU&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.btitle=FLUORESCENT%20ANTIBODY%20METHODS%20AS%20A%20HELP%20IN%20THE%20CLINICAL%20DIAGNOSIS%20OF%20STREPTOCOCCAL%20SORE%20THROAT&rft.au=SHUEY,H%20E&rft.aucorp=FITZSIMONS%20GENERAL%20HOSPITAL%20DENVER%20COLO&rft.date=1961-06-30&rft_id=info:doi/&rft_dat=%3Cdtic_1RU%3EAD0259859%3C/dtic_1RU%3E%3Cgrp_id%3Ecdi_FETCH-dtic_stinet_AD02598593%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true