STUDIES ON THE PURIFICATION OF VACCINIA VIRUS

Various methods are described and compared for removing extraneous matter from vaccinia virus suspensions. Of ten procedures tested, differential centrifugation and Freon extraction were the most successful. Both the partially purified suspensions and the dried products prepared from them had high v...

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Bibliographic Details
Main Authors: Orlando, Michael D, Riley, Jean M, Patrick, III, William C
Format: Report
Language:English
Subjects:
EMBRYONATED EGG TECHNIQUE
EXTRACTION
FREEZE DRYING
INFECTIOUS DISEASES
MICE
Microbiology
Pharmacology
PURIFICATION
VACCINIA VIRUS
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Summary:Various methods are described and compared for removing extraneous matter from vaccinia virus suspensions. Of ten procedures tested, differential centrifugation and Freon extraction were the most successful. Both the partially purified suspensions and the dried products prepared from them had high virus titers in embryonated eggs and high infectivity for mice. Although both processes resulted in products with the same titers, the differentially centrifuged product was approximately tenfold more purified as shown by the percentage solids of the suspensions before freeze drying.