EFFECT OF ISONICOTINYL HYDRAZIDE ON THE PATH OF CARBON IN PHOTOSYNTHESIS

In a study of the pathways of incorporation of carbon-14 from labelled carbon dioxide into the amino-acids glycine and serine during photosynthesis, the compound isonicotinyl hydrazide (isoniazid) was used. Isoniazid had only a small effect on the total fixation of carbon-14 and on the distribution...

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Bibliographic Details
Main Authors: PRITCHARD,G G, Whittingham,C P, Griffin,Wendy J
Format: Report
Language:English
Subjects:
ALGAE
AMINO ACIDS
CARBON
ENZYMES
GLYCINE
ISONIAZID
METABOLISM
ORGANIC PHOSPHORUS COMPOUNDS
PHOTOSYNTHESIS
SERINE
SUCROSE
TRACER STUDIES
UNITED KINGDOM
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Summary:In a study of the pathways of incorporation of carbon-14 from labelled carbon dioxide into the amino-acids glycine and serine during photosynthesis, the compound isonicotinyl hydrazide (isoniazid) was used. Isoniazid had only a small effect on the total fixation of carbon-14 and on the distribution between the ethanol-soluble and ethanolinsoluble fractions. The incorporation of carbon-14 into sugar phosphates, phosphoglyceric acid, phosphoenol pyruvic acid, aspartic acid, malic acid, fumaric acid and alanine is considerably reduced by treatment with isoniazid. Incorporation into sucrose is stimulated at the lower concentration and slightly inhibited at the higher concentration. Incorporation into glycollic acid and glycine, is stimulated about 5- to 8-fold by treatment with 0.01 M isoniazid. Incorporation into serine is inhibited to the same extent as that into sugar phosphates, phosphoglyceric acid, etc. The results do not indicate specific inhibition of transaminases by isoniazid but suggest a different site of action. (Extracted) Pub. in Nature (Gt. Brit.) v190 n4775 p553-4 May 6, 1961 (Copies available only to DDC users).