DIFFERENTIATION BETWEEN PROTEOLYTIC AND TAME ESTEROLYTIC ACTIVITY IN CROTALUS ATROX VENOM

Although Crotalus atrox venom can hydrolyze proteins and arginine esters, these activities are not due to a trypsin-like enzyme. Proteolytic and esterolytic activities have been differentiated by specific inhibition by such compounds as PMSF, DFP, EDTA and 2-mercaptoethanol, and by actual resolution...

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Bibliographic Details
Main Authors: Bobbitt,Jesse D, Reed,Donald E
Format: Report
Language:English
Subjects:
Biochemistry
CHROMATOGRAPHIC ANALYSIS
ENZYME INHIBITORS
ENZYMES
PEPTIDE HYDROLASES
PLASMIN
TRYPSIN
VENOMS
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Summary:Although Crotalus atrox venom can hydrolyze proteins and arginine esters, these activities are not due to a trypsin-like enzyme. Proteolytic and esterolytic activities have been differentiated by specific inhibition by such compounds as PMSF, DFP, EDTA and 2-mercaptoethanol, and by actual resolution of the venom by DEAE-cellulose chromatography. Preliminary results suggest that the proteolytic activity in the venom may have a limited specificity and that this specificity is quite unlike that of trypsin and plasmin. (Author)