The Use of a Carbocyanine Dye to Detect Trace Amounts of Protein in Biological Media

To test its applicability as a detector of biopolymers, a carbocyanine dye was allowed to react with proteins, polypeptides, polysaccharides, lipids, and supernatants from marine cultures, and changes in the visible spectra were observed. beta-Lactoglobulin and gelatin have thus far proved satisfact...

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Main Authors: Michajluk,Beverly Jae, Loeb,George I
Format: Report
Language:English
Subjects:
Biochemistry
BIOLOGICAL STAINS
CARBOHYDRATES
COLORIMETRIC ANALYSIS
CYANINE DYES
DYES
LIPIDS
MARINE BIOLOGY
METACHROMASIA
PEPTIDES
POLYMERS
PROTEINS
ULTRAVIOLET SPECTRA
VISIBLE SPECTRA
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Loeb,George I
description To test its applicability as a detector of biopolymers, a carbocyanine dye was allowed to react with proteins, polypeptides, polysaccharides, lipids, and supernatants from marine cultures, and changes in the visible spectra were observed. beta-Lactoglobulin and gelatin have thus far proved satisfactory as standards; the smallest detectable concentration was 2 ppm. Since different spectral features become prominent when the type of polymer or the state of the polymer is varied, it may be possible to classify marine polymers in very dilute solution by spectral means. Evidence has been found for considering a peak at 605 nm as characteristic of a denatured protein. (Author)
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Since different spectral features become prominent when the type of polymer or the state of the polymer is varied, it may be possible to classify marine polymers in very dilute solution by spectral means. Evidence has been found for considering a peak at 605 nm as characteristic of a denatured protein. 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source DTIC Technical Reports
subjects Biochemistry
BIOLOGICAL STAINS
CARBOHYDRATES
COLORIMETRIC ANALYSIS
CYANINE DYES
DYES
LIPIDS
MARINE BIOLOGY
METACHROMASIA
PEPTIDES
POLYMERS
PROTEINS
ULTRAVIOLET SPECTRA
VISIBLE SPECTRA
title The Use of a Carbocyanine Dye to Detect Trace Amounts of Protein in Biological Media
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