Activation of the Alternate Complement Pathway by Autologous Red Cell Stroma

The study demonstrates the ability of human autologous RBC stroma to activate the complement system via the recently described alternate pathway (C3-Activator System, Properdin System). Hemoglobin-free and hemoglobin-containing RBC stroma were prepared by osmotic lysis, repeated centrifugation, and...

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Bibliographic Details
Main Authors: Poskitt,Thomas R, Fortwengler,Philip , Jr, Lunskis,Beverly J
Format: Report
Language:English
Subjects:
BLOOD COAGULATION
COMPLEMENT(BIOLOGY)
ERYTHROCYTES
EXCITATION
HEMOLYSIS
Medicine and Medical Research
RED CELL STROMA
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Summary:The study demonstrates the ability of human autologous RBC stroma to activate the complement system via the recently described alternate pathway (C3-Activator System, Properdin System). Hemoglobin-free and hemoglobin-containing RBC stroma were prepared by osmotic lysis, repeated centrifugation, and ultrasound sonication. Samples of RBC ghosts, sonicated ghosts, intact RBCs, hemoglobin, and concentration platelet stroma were incubated with autologous serum and the cleavage of C3-Activator (C3A) from C3-Proactivator (C3PA) determined immunoelectrophoretically. Autologous RBC ghosts and stroma were equally effective in generating C3A whereas intact RBCs, platelet stroma, and hemoglobin were inactive. It is postulated that this in vitro activity of RBC stroma may occur intravascularly when erythrocytes are damaged by immune or nonimmune mechanisms and, through an interaction with platelets, may constitute a mechanism for activation of the coagulation system during acute hemolytic episodes. (Author)