A Rapid Method for Determining the Percentage of Antibacterial Phagocytes in a Sample Population of Leukocytes. (Assay of Antibacterial Phagocyte Populations)

A relatively rapid, simple direct microscopic assay of the antibacterial capability of phagocyte populations has been developed. Normal mice or mice treated with 7.6% sodium caseinate to stimulate production of peritoneal exudate cells were inoculated intraperitoneally with actively growing Vibrio p...

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Bibliographic Details
Main Authors: Janssen,Werner A, Dangerfield,Harry G
Format: Report
Language:English
Subjects:
AS841
BACTERIAL DISEASES
IN VIVO ANALYSIS
INCUBATION
LEUKOCYTES
Medicine and Medical Research
PE62776A
PERITONEUM
PHAGOCYTES
POLYMORPHONUCLEAR NEUTROPHILS
RESISTANCE(BIOLOGY)
TEST METHODS
THERAPY
VIBRIO
Vibrio Parahemolyticus
WU005
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Summary:A relatively rapid, simple direct microscopic assay of the antibacterial capability of phagocyte populations has been developed. Normal mice or mice treated with 7.6% sodium caseinate to stimulate production of peritoneal exudate cells were inoculated intraperitoneally with actively growing Vibrio parahemolyticus. Thirty minutes later phagocytes were harvested by washing the peritoneal cavity. The antibacterial potential of the phagocyte population was determined by comparing the percentage of phagocytes with ingested vibrios before culture with the percentage of phagocytes whose intracellular V. parahemolyticus failed to replicate after incubation. Essentially all phagocytic cells in peritoneal washings from normal mice were found to be macrophages, less than 50% of which had antibacterial activity against V. parahemolyticus. Antibacterial phagocyte populations were greatly increased following exudate induction; within 4 hr the exudates contained both neutrophils and macrophages, and essentially all phagocytizing cells had effective antibacterial capability.