Two Novel Methods for the Preparation of Erythrocyte Stroma Free from Haemoglobin Compared to the Conventional Centrifugation Technique

The removal of haemoglobin from haemolysed erythrocyte stroma by washing over a membrane filter or by passage through a column of porous gel was compared to the removal achieved by washing in the centrifuge. The column method was preferable for an initial rapid clean-up, which could be followed by a...

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Bibliographic Details
Main Authors: Crone,H D, Poretski,M, Bladen,M P
Format: Report
Language:English
Subjects:
Anatomy and Physiology
Blood
Buffers(Chemistry)
Centrifuge separation
Cleaning
Efficiency
Erythrocytes
Filtration
Gels
Hemoglobin
Hemolysis
Histological techniques
Laboratory procedures
Membranes(Biology)
Microbiology
Solutions(General)
Test Facilities, Equipment and Methods
Timeliness
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Summary:The removal of haemoglobin from haemolysed erythrocyte stroma by washing over a membrane filter or by passage through a column of porous gel was compared to the removal achieved by washing in the centrifuge. The column method was preferable for an initial rapid clean-up, which could be followed by a further pass through the column, or by centrifugation to concentrate the suspension. Membrane filtration was useful when total recovery of a small sample of storma was required.