Choline Uptake by Glomerular Synapses Isolated from Bovine Cerebellar Vermis

Tritium Choline uptake was investigated in a highly enriched preparation of glomerular particles isolated from bovie cerebellar vermis. Kinetic analysis indicates that a high affinity uptake sytstem with a relatively low maximum uptake velocity is present. At a substrate concentration of .0000001 M,...

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Bibliographic Details
Main Authors: Terrian,D M, Noisin,E L, Thomas,W E
Format: Report
Language:English
Subjects:
Anatomy and Physiology
Biochemistry
BIOLOGICAL ABSORPTION
BOVINES
CEREBELLUM
CHOLINERGIC NERVES
CHOLINES
CONCENTRATION(COMPOSITION)
ENRICHMENT
GLOMERULI
KINETICS
NERVE FIBERS
PARTICLES
PE61102F
PREPARATION
REPRINTS
SUBSTRATES
SYNAPSE
TRITIUM
WUAFOSR2312V401
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Summary:Tritium Choline uptake was investigated in a highly enriched preparation of glomerular particles isolated from bovie cerebellar vermis. Kinetic analysis indicates that a high affinity uptake sytstem with a relatively low maximum uptake velocity is present. At a substrate concentration of .0000001 M, Tritium choline uptake was shown to be sodium-dependent, hemicholinium-sensitive and nonreactive to increasing concentrations of unlabeled choline. These findings support the presence of a high affinity choline uptake system in cerebellar glomeruli and would seem to be consistent with the suggestion that a small proportion of mossy fibers are cholinergic in this brain region. Pub. in Brain Research, v336 p401-404 1986.