Ultrastructural and Functional Effects of Lipopolysaccharide and Interleukin-2 on Human NK Cells

Bacterial endotoxin (lipopolysaccharide, LPS) and interleukin-2 (IL- 2) are know to stimulate NK cell mediated cytotoxicity against tumor cells. In the present report we sought to correlate the stimulatory effect of LPS and IL-2 on NK cell activity with ultrastructural changes which occurred as a re...

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Bibliographic Details
Main Authors: Kang, Yuan-Hsu, Carl, Mitchell, Watson, Lorrita P
Format: Report
Language:English
Subjects:
Anatomy and Physiology
BACTERIA
BLOOD CELLS
CELLS(BIOLOGY)
CYTOCHEMISTRY
CYTOTOXINS
ENDOTOXINS
INTERLEUKIN-2
LIPOPOLYSACCHARIDES
LYMPHOCYTES
NATURAL KILLER CELLS
NEOPLASMS
NK CELLS
NUCLEI
PE61153N
PHARMACOKINETICS
Pharmacology
PRODUCTION
REPRINTS
RESPONSE(BIOLOGY)
WUDN247511
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Summary:Bacterial endotoxin (lipopolysaccharide, LPS) and interleukin-2 (IL- 2) are know to stimulate NK cell mediated cytotoxicity against tumor cells. In the present report we sought to correlate the stimulatory effect of LPS and IL-2 on NK cell activity with ultrastructural changes which occurred as a result of such stimulation. Peripheral blood mononuclear cells (PBMC) were purified from healthy donors by a Ficoll-Hypaque density gradient technique. Results showed that both LPS and IL-2 significantly enhanced the cytotoxic activity of PBMC. These data indicate that: (1) the effect of LPS on the enhancement of NK cytotoxicity in PBMC may be a direct and/or indirect process involving production of lymphokines; (2) LPS has a direct effect on sorted Leu-11a(+) cells (3) IL-2 stimulates mitosis of Leu-7+ cells and Leu-11a+ cells; and (4) the LPS or Il-2 induced ultrastructural changes in Leu-11a+ cells are consistent with the enhanced NK cytotoxicity. Pub. in Scanning Microscopy, v2, n3, p1567-1586, 1988.