Immunological Approach to the Identification and Development of Vaccines to Various Toxins

Monoclonal antibodies specific for the sodium channel blockers saxitoxin (STX) and tetrodotoxin (TDT) have been generated and characterized as to their abilities to protect against toxin-induced in vitro toxicity and to induce anti-idiotypic immune responses. In a continued effort to enhance the bin...

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Bibliographic Details
Main Authors: Chanh, Tran C, Armstrong, Deborah, Kennedy, Ronald
Format: Report
Language:English
Subjects:
ANTIBODIES
ANTIIDIOTYPIC VACCINES
ASS12
CLONES
FUNCTIONS
IMMUNIZATION
IMMUNOLOGY
IN VITRO ANALYSIS
Medicine and Medical Research
MICE
MONOCLONAL ANTIBODIES
NERVES
PE61102A
Pharmacology
SAXITOXIN
STIMULATION(PHYSIOLOGY)
TEST AND EVALUATION
TETRODOTOXIN
TOXICITY
Toxicology
TOXINS AND ANTITOXINS
VACCINES
WU126
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Summary:Monoclonal antibodies specific for the sodium channel blockers saxitoxin (STX) and tetrodotoxin (TDT) have been generated and characterized as to their abilities to protect against toxin-induced in vitro toxicity and to induce anti-idiotypic immune responses. In a continued effort to enhance the binding affinities of the anti-toxin monoclonal antibodies, two novel approaches have been undertaken. The first one involves the design of a new toxin conjugate for use in immunization of BALB/c mice. The second approach involves the in vitro stimulation with free STX or TDT of mouse spleen cells hyper-immunized in vivo. Monoclonal anti-TDT and anti-STX antibodies have been generated that are protective against TDT and STX toxicity, respectively, on nerve functions. Rabbit anti-idiotypic antibodies specific for TD2C5 anti-TDT have been generated, purified and used in immunization of mice in order to test the potential use of anti-idiotype as a vaccine in inducing a systemic anti-TDT immune response. Keywords: Monoclonal antibodies, In situ protection, Anti- idiotypic vaccines.