Isolation of Salmonella typhi from Standard Whole Blood Culture versus Blood-Clot Cultures

The use of 10% oxgall and bile broth medium, both supplemented with freshly prepared 100 u/ml streptokinase, for isolating Salmonella typhi by clot culture technique was evaluated and compared against whole blood culture systems (3 ml blood in 9 ml media and 8 ml blood in 72 ml media). These gave a...

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Bibliographic Details
Main Authors: Tjaniadi, Periska, Lane, Edward M, Lesmana, Murad, Edman, David C, Kostermans, Deskian
Format: Report
Language:English
Subjects:
BILE
Biology
BLOOD
CLOT CULTURES
COAGULATION
CULTURE
CULTURES(BIOLOGY)
ISOLATION
Microbiology
PE63706N
RATES
RATIOS
REPRINTS
SALMONELLA TYPHOSA
SOUP
Test Facilities, Equipment and Methods
WHOLE BLOOD CULTURES
WU5037
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Summary:The use of 10% oxgall and bile broth medium, both supplemented with freshly prepared 100 u/ml streptokinase, for isolating Salmonella typhi by clot culture technique was evaluated and compared against whole blood culture systems (3 ml blood in 9 ml media and 8 ml blood in 72 ml media). These gave a 1:4 and 1:10 blood to medium ratio, respectively. Clot cultures in 10% oxgall (CLOX) gave a 57% positive isolation rate for S. typhi. A similar result was obtained from clot cultures in bile broth medium (CLBB). A total of 184 samples identified as positive for S. typhi were tested. There was no significant difference between the use of 10% oxgall or bile broth medium when used for clot culture. The whole blood culture systems still showed a significantly better rate of isolation than the clot culture methods. Keywords: Cultures biology; Clot cultures; Salmonella typhi; Isolation of S. typhi; Whole blood culture; Blood-clot culture; Reprints. Pub. in Jnl. of Southeast Asian Jnl. of Tropical Medicine and Public Health, v19 n4 p623-627 Dec 1988.