Flow Cytometric Ploidy Determination of Oral Premalignant and Malignant Lesions

Nuclear DNA content was evaluated for use as an objective parameter of diagnostic value in oral premalignancy and malignancy. Fifty-three blocks of formalin-fixed and paraffin-embedded archival tissue were selected from 20 cases which has been diagnosed as premalignant epithelial lesions and subsequ...

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Bibliographic Details
Main Author: Pemble, III, Charles W
Format: Report
Language:English
Subjects:
CANCER
CELLS
COEFFICIENTS
DETERMINATION
DIAGNOSIS(GENERAL)
MEDICINE
Medicine and Medical Research
PARAMETERS
SUSPENSION DEVICES
VARIATIONS
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Summary:Nuclear DNA content was evaluated for use as an objective parameter of diagnostic value in oral premalignancy and malignancy. Fifty-three blocks of formalin-fixed and paraffin-embedded archival tissue were selected from 20 cases which has been diagnosed as premalignant epithelial lesions and subsequently diagnosed as having progressed to malignancy. A single cell suspension was prepared from each tissue block, stained with propidium iodide and subjected to flow cytometric analysis. This yielded histograms which depicted the ploidy status for each specimen. For five specimens, the tissue quantity was insufficient and for an additional six specimens, the coefficient of variation for the histogram exceeded the established limit of seven.