Hypertonic Saline Dextran: Immunological and Hematological Effects Following Single and Multiple Doses in Dogs and Pigs

Dextran has been used in resuscitation from hypovolemia and trauma for the past 40 years. This project examined the effects of multiple doses of 20 ml/kg body weight Hypertonic Saline Dextran-70 on the immune system in dogs and the effects of a single dose of 4 ml/kg body weight on blood aggregation...

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Bibliographic Details
Main Authors: Kilani, Ahmed F, Summary, James J, Dubick, Michael A
Format: Report
Language:English
Subjects:
ACTIVATION
BLOOD
BLOOD VOLUME
COAGULATION
COLLAGEN
DEXTRAN
DEXTRAN-70
DOGS
DOSAGE
EPINEPHRINE
FORMULATIONS
HEMOSTASIS
HYPERTONIC
HYPERTONIC RESUSCITATION
IMMUNITY
IMMUNOASSAY
IMMUNOGLOBULINS
LOW LEVEL
Medicine and Medical Research
PRECIPITATION
PROTHROMBIN
RESUSCITATION
SALINITY
THROMBOPLASTIN
TIME
TRAUMA
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Summary:Dextran has been used in resuscitation from hypovolemia and trauma for the past 40 years. This project examined the effects of multiple doses of 20 ml/kg body weight Hypertonic Saline Dextran-70 on the immune system in dogs and the effects of a single dose of 4 ml/kg body weight on blood aggregation and clot formation in pigs. The project focused on the use of a new dextran formula, Hypertonic Saline Dextran, HSD (6% Dextran-70 in 7.5% NaCl). Immunoprecipitation experiments using specimens from 24 Beagle dogs revealed no significant changes in the levels of IgG, IgM, and C3 protein following daily infusions of HSD at doses up to five times the recommended dose. Following a single HSD infusion of the recommended dose of 4 ml/kg body weight, specimens from 21 pigs revealed no significant changes in prothrombin time (PT) and activated partial thromboplastin time (APTT) throughout the testing period of 168 hours. Following HSD infusion in 7 of these pigs, platelet aggregation was tested using the agonists ADP, collagen, epinephrine and ristocetin. ADP was the only agonist that caused a slight increase in the percent platelet aggregation.