Interactions Between HIV-Infected Monocytes and the Extracellular Matrix: HIV-Infected Monocytes Secrete Neutral Metalloproteases That Degrade Basement Membrane Protein Matrices

The frequency of human immunodeficieny virus (HIV)-infected monocytes that spread on a model basement membrane was about twofold greater than that of a n equal number of uninfected control cells through the initial 12 to 18 h of culture. By 24 h, virtually all HIV-infected and uninfected control cel...

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Bibliographic Details
Main Authors: Dhawan, Subhash, Toro, Luis A, Jones, B E, Meltzer, Monte S
Format: Report
Language:English
Subjects:
ACQUIRED IMMUNE DEFICIENCY SYNDROME
BASEMENT MEMBRANE
BASEMENTS
Biochemistry
CARCINOGENESIS
CELLS
CELLULAR
CONTRAST
CONTROL
CULTURE
CULTURES(BIOLOGY)
FLUIDS
FREQUENCY
GELS
Genetic Engineering and Molecular Biology
HUMAN IMMUNODEFICIENCY VIRUSES
HUMANS
INFECTIOUS DISEASES
INFECTIOUS MONONUCLEOSIS
INTERVALS
LEUKOCYTES
MEMBRANES
METALLOPROTEASES
METALS
Microbiology
MOLECULAR BIOLOGY
MONOCYTES
NETWORKS
NEUROPATHOLOGY
NEUTRAL
NUMBERS
ONCOGENESIS
PEPTIDE HYDROLASES
POPULATION
SECRETION
TIME
TIME INTERVALS
VIRUS DISEASES
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Summary:The frequency of human immunodeficieny virus (HIV)-infected monocytes that spread on a model basement membrane was about twofold greater than that of a n equal number of uninfected control cells through the initial 12 to 18 h of culture. By 24 h, virtually all HIV-infected and uninfected control cells spread on the basement membrane gel. The frequency of spread cells in the uninfected control population was 10% of total cells by 12 days. In contrast, 30 to 40% of HIV-infected monocytes remained spread through this time interval and formed a dense interdigitated network of cell processes on and into the gel matrix. Invasion of the basement membrane matrix by HIV-infected monocytes suggested increased secretion of proteases able to digest the gel. Indeed, levels of neutral protease activity in culture fluids from HIV-infected monocytes were significantly higher than those from equal numbers of uninfected control cells. High levels of protease activity in culture fluids of HIV-infected monocytes required productive virus infection and were not observed with cells exposed to T cell-tropic HIV isolates. The predominant protease activity in these cultures was a 92-kd neutral metallogelatinase. HIV-induced changes in monocyte metalloprotease activity may be important for extravasation of infected cells to tissue or for the development of AIDS-associated neuropathology, carcinogenesis, and opportunistic infection.HIV-1, Metalloproteases, Basement membrane, Extracellular matrix. Original contains color plates: All DTIC reproductions will be in black and white. Pub. in Jnl. of Leukocyte Biology, v52 n2 p244-248 Aug 92.