Screening Test and Isolation Procedure for TNT-Degrading Microorganisms

A test was developed to screen soils and sediments for the presence of microbial activity against 2,4,6-trinitrotoluene (TNT). TNT removal was observed with and without the use of chemicals to stimulate microbial activity., In addition to a small amount of TNT added to each soil sample, chemical tre...

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Bibliographic Details
Main Authors: Gunnison, Douglas, Pennington, Judith C, Price, Cynthia B, Myrick, Glenn B
Format: Report
Language:English
Subjects:
ACETATES
AGAR
Ammunition and Explosives
ANILINES
BIODETERIORATION
CHEMICALS
CONTAMINANTS
DEGRADATION
EXPLOSIVES
GLUCOSE
INCUBATION
INORGANIC COMPOUNDS
ISOLATION
METABOLITES
MICROORGANISMS
MINERALIZATION
MOLECULAR STRUCTURE
NITRO RADICALS
NITROPHENOLS
OBSERVATION
ORGANIC COMPOUNDS
Physical Chemistry
PSEUDOMONAS
REMOVAL
SALTS
SAWDUST
SEDIMENTS
SLURRIES
SODIUM
Soil Mechanics
SOILS
STATICS
SUBSTRATES
SUCCINATES
TEST AND EVALUATION
TNT
TOLUENES
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Summary:A test was developed to screen soils and sediments for the presence of microbial activity against 2,4,6-trinitrotoluene (TNT). TNT removal was observed with and without the use of chemicals to stimulate microbial activity., In addition to a small amount of TNT added to each soil sample, chemical treatments included toluene, dinitroaniline, dinitro-o-cresol, dinitrophenol, sodium acetate, sawdust, or sodium succinate. Following 1 to 4 weeks of incubation in static and slurry modes, individual soil treatments were plated onto a crystalline lawn of TNT overlying a basal salts agar containing one of three cometabolites (acetate, glucose, or succinate). Activity against TNT was detected by visual observation of TNT clearing around the soil sample. The screening procedure was applied to soils from seven military installations. Positive results were obtained for each soil, although the amount of activity varied from soil to soil. Based on these results, the following combinations of screening substrates and cometabolites are recommended: (a) acetate and succinate as combined priming substrates and cometabolites and (b) toluene, dinitroaniline, and dinitro-o-cresol as priming substrates with acetate, succinate, and glucose as cometabolites. Biodegradation, Mineralization, Biotransformation, Phenaerochaete, Biotreatment, Pseudomonas, Explosive, Screening test, Microorganism.