Role of Plasminogen Activator Inhibitor-1 in Promoting Fibrin Deposition in Rabbits Infused with Ancrod or Thrombin

The role of defective, fibrinolysis caused by elevated activity of plasminogen activator inhibitor-1 (PAI-1) in promoting fibrin deposition in vivo has not been well established. The present study compared the efficacy of thrombin or ancrod, a venom-derived enzyme that clots fibrinogen, to induce fi...

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Main Authors: Krishnamurti, Chitra, Bolan, Charles, Colleton, Curtis A, Reilly, Thomas M, Alving, Barbara M
Format: Report
Language:English
Subjects:
ANIMALS
Biochemistry
BLOOD COAGULATION
DEPOSITION
ENDOTOXINS
ENZYME PRECURSORS
ENZYMES
FIBRIN
FIBRINOGEN
FIBRINOLYSIS
HISTOPATHOLOGY
HUMANS
IN VIVO ANALYSIS
INFUSIONS
INHIBITORS
MALES
NEW ZEALAND
PHYSIOLOGY
RABBITS
REPRINTS
THROMBIN
VENOMS
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creator Krishnamurti, Chitra
Bolan, Charles
Colleton, Curtis A
Reilly, Thomas M
Alving, Barbara M
description The role of defective, fibrinolysis caused by elevated activity of plasminogen activator inhibitor-1 (PAI-1) in promoting fibrin deposition in vivo has not been well established. The present study compared the efficacy of thrombin or ancrod, a venom-derived enzyme that clots fibrinogen, to induce fibrin formation in rabbits with elevated PAI-1 levels. One set of male New Zealand rabbits received intravenous endotoxin to increase endogenous PAI-1 activity followed by a 1 -hour infusion of ancrod or thrombin; another set of normal rabbits received intravenous human recombinant PAI-1 (rPAI-1) during an infusion of ancrod or thrombin. Thirty minutes after the end of the infusion, renal fibrin deposition was assessed by histopathology. Animals receiving endotoxin, rPAI-1 , ancrod, or thrombin alone did not develop renal thrombi. Pub. in Blood, v82, n12, p3631-3636, 15 Dec 1993.
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The present study compared the efficacy of thrombin or ancrod, a venom-derived enzyme that clots fibrinogen, to induce fibrin formation in rabbits with elevated PAI-1 levels. One set of male New Zealand rabbits received intravenous endotoxin to increase endogenous PAI-1 activity followed by a 1 -hour infusion of ancrod or thrombin; another set of normal rabbits received intravenous human recombinant PAI-1 (rPAI-1) during an infusion of ancrod or thrombin. Thirty minutes after the end of the infusion, renal fibrin deposition was assessed by histopathology. Animals receiving endotoxin, rPAI-1 , ancrod, or thrombin alone did not develop renal thrombi. 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source DTIC Technical Reports
subjects ANIMALS
Biochemistry
BLOOD COAGULATION
DEPOSITION
ENDOTOXINS
ENZYME PRECURSORS
ENZYMES
FIBRIN
FIBRINOGEN
FIBRINOLYSIS
HISTOPATHOLOGY
HUMANS
IN VIVO ANALYSIS
INFUSIONS
INHIBITORS
MALES
NEW ZEALAND
PHYSIOLOGY
RABBITS
REPRINTS
THROMBIN
VENOMS
title Role of Plasminogen Activator Inhibitor-1 in Promoting Fibrin Deposition in Rabbits Infused with Ancrod or Thrombin
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