Metabolism of 2,2-Dichloro-1,1,1 Trifluoroethane (HCFC-123) by Human Hepatic Microsomes

As part of its safety evaluation, the in vitro metabolism of 2,2-dichloro-1,1,l-trifluoroethane (HCFC-123), a replacement candidate for Halon 1211, by human hepatic microsomes, was assessed. Microsomal incubations containing HCFC-123 ranging from 6 to 75% (v/v) in the headspace produced increasing a...

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Bibliographic Details
Main Authors: Godlin, C. S, Ketcha, M. M, Drerup, J. M, Vinegar, A
Format: Report
Language:English
Subjects:
1-DICHLORO-2
2-TRIFLUOROETHANE
Anatomy and Physiology
Biochemistry
HALOCARBON
HUMAN METABOLISM
HUMANS
IN VITRO ANALYSIS
INCUBATION
INHIBITION
LIVER
METABOLISM
MICROSOMES
OPTIMIZATION
PE62202F
PH FACTOR
PHYSIOLOGY
SAFETY
STEADY STATE
SUBSTRATES
TEST AND EVALUATION
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Summary:As part of its safety evaluation, the in vitro metabolism of 2,2-dichloro-1,1,l-trifluoroethane (HCFC-123), a replacement candidate for Halon 1211, by human hepatic microsomes, was assessed. Microsomal incubations containing HCFC-123 ranging from 6 to 75% (v/v) in the headspace produced increasing amounts of trifluoroacetic acid (IFA); the kinetics suggested substrate- saturation although substrate inhibition was apparent above a concentration of 36%. The rate of ThA formation with respect to pH, time, and protein concentration permitted linear rates of formation to be determined. Rates of ThA formation from incubations conducted at physiological pH, and containing concentrations of HCFC-123 in solution representing estimated concentrations of the chemical in human liver at steady-state, were 67% of those obtained under optimal conditions.