Response of Artificial Human Skin to Irritants: Cytokine and Prostaglandin Release

Cytokines have been implicated in aspects of vesicant injury/repair. This study describes responses of artificial human skin (Skin2 and EpiDerm) to chloroethyl ethyl sulfide (CEES), defined by interleukin-la (Tb-la), tumor necrosis factor-a (TNF-a) and prostaglandin E2 (PGE2) release. Skin2 and EpiD...

Full description

Saved in:
Bibliographic Details
Main Authors: Bowers, W., Jr, Blaha, M, Alkhyyat, A, Walker, J
Format: Report
Language:English
Subjects:
ARTIFICIAL TISSUE
ASSAYING
BURNS(INJURIES)
CEES(CHLOROETHYL ETHYL SULFIDE)
CHLORINE COMPOUNDS
CONTROL
CYTOKINE
ELISA(ENZYME LINKED IMMUNOSORBENT ASSAYS)
ETHYL RADICALS
FIBROBLASTS
FLOW RATE
HUMANS
IRRITATING AGENTS
MEDIA
MEDIA(MEDICINE)
Medicine and Medical Research
Pharmacology
PROSTAGLANDIN
RELEASE
REPAIR
RESISTANCE(BIOLOGY)
SKIN(ANATOMY)
SULFIDES
THAWING
TISSUES(BIOLOGY)
TNF ALPHA(TUMOR NECROSIS FACTOR ALPHA)
VESICANTS
WOUNDS AND INJURIES
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cytokines have been implicated in aspects of vesicant injury/repair. This study describes responses of artificial human skin (Skin2 and EpiDerm) to chloroethyl ethyl sulfide (CEES), defined by interleukin-la (Tb-la), tumor necrosis factor-a (TNF-a) and prostaglandin E2 (PGE2) release. Skin2 and EpiDerm in Millicells of 6 well Costar trays containing 1ml of assay media/well were exposed to CEES (2.Omg/L, flow rate 1l/min for 2hr) in humidified air. Control tissues were exposed without CEES. Millicells containing Skin2 or EpiDerm (12/group) were transferred to fresh assay media and incubated for 22 hr. Tissues (6/group) were used for MTT tests. Media from each well were stored in liquid N2. Tb-la (RIA or EbISA), PGE2 (RIA or ETA), and TNF-a (ETA) were measured in thawed specimens. CEES significantly increased release of Tb-la (l92pg/ml plus or minus 34.9, control SSpg/ml i 16.6) and PGE2 (3,977pg/O.lml plus oir minus 1,197, control 2154lpg/O.lml plus or minus 570) from Skin21 but not TNF-a levels, with viability (MTT) 3%. Neither Tb-la nor TNF-a were elevated by CEES-exposed EpiDerm, although PGE2 was elevated (25Bpg/O.lml plus or minus 71 vs 184 plus or minus 79), viability 46%. We conclude pro-inflammatory mediators, Tb-la and PGE21 could play significant roles in CEES injury and that either fibroblasts are critical to the process, or EpiDerm, which lacks fibroblasts, is somehow more resistant.