Complement Activation By Liposome-Encapsulated Hemoglobin in Vitro: The Role of Endotoxin Contaminative

Incubation of liposome-encapsulated Hb (LEH) with rat serum at 37C led to accelcelerated decay of serum complement (C) hemolytic activity (CH50). Empty liposomes (L) caused less expressed decrease of CH50, whereas free Hb had no effect on C hemolytic activity. The LEH- and L-induced increase in C co...

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Bibliographic Details
Main Authors: Szebeni, Janos, Wassef, Nabila M, Rudolph, Alan S, Alving, Carl R
Format: Report
Language:English
Subjects:
Biochemistry
BLOOD SERUM
CONSUMPTION
CONTAMINATION
ENCAPSULATION
ENDOTOXINS
HEMOGLOBIN
HEMOLYSIS
IN VITRO ANALYSIS
LEH(LIPOSOME ENCAPSULATED HEMOGLOBIN)
LIPOSOMES
Medicine and Medical Research
RATS
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Summary:Incubation of liposome-encapsulated Hb (LEH) with rat serum at 37C led to accelcelerated decay of serum complement (C) hemolytic activity (CH50). Empty liposomes (L) caused less expressed decrease of CH50, whereas free Hb had no effect on C hemolytic activity. The LEH- and L-induced increase in C consumption was unlikely due to endotoxin (LPS) contamination, as spiking of rat serum with LPS caused reduction in CH50 only at levels significantly higher than detectable in LEH or L. The LPS- induced C consumption was not potentiated by free hemoglobin.