Modifying Hydroxyapatite Nucleating Peptides to Form Novel Boron Ceramics

Reported are efforts to modify the Hydroxyapatite nucleating peptide (HANP) isolated from Corynebacterium matruchotti to nucleate boron as opposed to calcium. The literature reports that boron-binding proteins utilize a His and Ser complex to bind boron in their active sites. Using computer modeling...

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Bibliographic Details
Main Author: Stote, Robert E , II
Format: Report
Language:English
Subjects:
BORON
CALCIUM
CERAMIC MATERIALS
CLONES
Coatings, Colorants and Finishes
COMPUTERIZED SIMULATION
CORYNEBACTERIUM
DOCUMENTS
HANP(HYDROXYAPATITE NUCLEATING PEPTIDE)
HYDROXYAPATITE NUCLEATING PEPTIDE
Inorganic Chemistry
NUCLEATION
Organic Chemistry
PE61102A
PEPTIDES
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Summary:Reported are efforts to modify the Hydroxyapatite nucleating peptide (HANP) isolated from Corynebacterium matruchotti to nucleate boron as opposed to calcium. The literature reports that boron-binding proteins utilize a His and Ser complex to bind boron in their active sites. Using computer modeling, modifications were designed that changed the active site of HANP to include two His, two Ser or a Ser and a His. Four modified versions were proposed and each version was tested for their energy minimization. The computer models indicate the structures of all 4 were stable. The modified versions were cloned into E. coli and expressed. The expressed peptides were then tested for their ability to nucleate boron ceramics with no success. Two of the modified peptides were synthesized chemically and examined using circular dichromism(CD) and nuclear magnetic reasonance. The results suggest that the a-helical structure of HANP is lost with the modifications. The results from these experiments will be presented in detail and their implications discussed.