Landscape Phage Probes for Salmonella Typhimurium

We selected from a landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for Salmonella typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA) and the...

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Bibliographic Details
Main Authors: Olsen, Eric V, Sorokulova, Iryna B, Chen, I-Hsuan, Fiebor, Ben, Barbaree, James M
Format: Report
Language:English
Subjects:
BACTERIOPHAGES
BIOSORBENT
CELLS(BIOLOGY)
DETECTION
ELECTRON MICROSCOPY
ENTEROBACTERIACEAE
ENZYMES
FLUORESCENCE
IMMUNOASSAY
LANDSCAPE PHAGE
Microbiology
MONITORING
OPTICAL ANALYSIS
PHAGE DISPLAY
PROBE
PROBES
SALMONELLA TYPHIMURIUM
TEST AND EVALUATION
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Summary:We selected from a landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for Salmonella typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA) and the precipitation test. Interaction of representative probes with Salmonella typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of highly specific and robust probes for Salmonella typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.