Generation Sampling and Analysis for Low-Level GB (SARIN) Vapor for Inhalation Toxicology Studies

• Main Authors: Muse, Jr , William T, Thomson, Sandra A, Matson, Kathy L, Crouse, Charles L
• Format: Report
• Language: English
• Subjects: Anatomy and Physiology; ATOMIZATION; CHEMICAL WARFARE AGENTS; Chemical, Biological and Radiological Warfare; CONCENTRATION(CHEMISTRY); GB AGENT; INHALATION; METHODOLOGY; SATURATION; SUBLETHAL DOSAGE; TOXICITY; Toxicology

This study tested and optimized various methodologies to generate, sample, and characterize sarin (GB) test atmospheres in an inhalation chamber, particularly at low-vapor levels. A syringe drive/spray atomization system produced GB vapor at lethal concentrations of 1-44 mg/m3. A saturator cell was used to generate GB vapor at sub-lethal concentrations from 1 mg/m down to very low GB levels approaching the TLV-TWA of 0.0001 mg/m3. Both generation techniques demonstrated the ability to produce stable vapor concentrations over extended exposure periods. This capability was important to determine sub-lethal nerve agent effects for inhalation toxicology studies. In addition, the techniques employed for producing and maintaining low-level GB vapor would lay the foundation for testing other chemical warfare agents, such as GF or VX. Prepared in collaboration with Geo-Centers Inc, Aberdeen Proving Grounds, MD.