Microarray Analysis of Transposon Insertion Mutants in Bacillus Anthracis: Global Identification of Genes Required for Sporulation and Germination

A transposon site hybridization (TraSH) assay was developed for functional analysis of the B. anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of the pathogen's genes. The system, used to identify genes required for generation of the infectious anthrax spore, spore...

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Bibliographic Details
Main Authors: Day , Jr , William A, Rasmussen, Suzanne L, Carpenter, Beth M, Peterson, Scott N, Friedlander, Arthur M
Format: Report
Language:English
Subjects:
ANTHRAX
BACILLUS ANTHRACIS
FUNCTIONAL ANALYSIS
GENES
GENOME
GERMINATION
GLOBAL
GROWTH(GENERAL)
HYBRIDIZATION
IDENTIFICATION
INFECTIOUS DISEASES
Medicine and Medical Research
MICROARRAYS
Microbiology
MUTAGENESIS
OPTIMIZATION
PATHOGENIC MICROORGANISMS
PHENOTYPE EXPRESSION
SOLID WASTES
SPORES
SPORULATION
TRANSPOSON INSERTION MUTANTS
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Description
Summary:A transposon site hybridization (TraSH) assay was developed for functional analysis of the B. anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of the pathogen's genes. The system, used to identify genes required for generation of the infectious anthrax spore, spore germination and optimal growth on rich medium, was predictive of the contribution of two conserved hypothetical genes for the phenotypes examined. The original document contains color images. Pub. in Journal of Bacteriology, v189 n8, p3296-3301, Apr 2007.