UF Biomotor/Biosensor Nanotechnologies

This report contains a summary of activities on the project UF Biomotor/Biosensor Nanotechnologies. Nanoscale actuators for use as molecular shuttles in biosensing devices were developed based on actin filament end-tracking motors. Novel strategies were developed exploiting these motors and modified...

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Bibliographic Details
Main Authors: Dickinson, Richard B, Purich, Daniel L, McGuire, Gary, Holiday, Brian, Wirtz, Denis, Cooke, William, Zeile, William, Hens, Suzanne, Interliggi, Kimberly, Sturm, Colin
Format: Report
Language:English
Subjects:
8MICRO/NANO-FABRICATION
ACTUATORS
Biochemistry
BIOMOLECULAR MOTORS
BIOMOLECULES
CELLS(BIOLOGY)
Electrical and Electronic Equipment
FILAMENT END-TRACKING MOTORS
MASS SPECTROMETRY
MATHEMATICAL MODELS
METHODOLOGY
NANOSCALE ACTUATORS
NANOTECHNOLOGY
NEXT-GENERATION BIOSENSING MICRODEVICES
PARTICLE PROPULSION
PE0602712E
POLYMERIZATION
PROPULSION SYSTEMS
PROTEINS
REAL TIME
VALIDATION
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Summary:This report contains a summary of activities on the project UF Biomotor/Biosensor Nanotechnologies. Nanoscale actuators for use as molecular shuttles in biosensing devices were developed based on actin filament end-tracking motors. Novel strategies were developed exploiting these motors and modified substrata to propel and guide motor-coated micro- and nanoparticles using substratum-bound actin filaments with their elongating plus-ends bound to the particle surface. Key accomplishments of this project include (1) optimization of conditions for particle propulsion in cell extracts, (2) development of single-filament actuators, (3) guidance of single-filament elongation on patterned and microfabricated substrata, (4) development and validation of a mathematical model that predict particle propulsion velocity as a function of controllable parameters, (5) novel time-of-flight mass spectrometry methods to image surfaces, and (6) direct real-time methods to observe protein-protein interactions involved in filament end-tracking in vivo. Prepared in cooperation with International Technology Center, Research Triangle Park; The College of William and Mary, Williamsburg, VA; and Johns Hopkins Univ., Baltimore, MD.