Dynamic Testing of Signal Transduction Deregulation During Breast Cancer Initiation

The goal of our study is to develop nanosensors to detect pre-cancerous changes in the breast and figure out which pre-cancerous breast changes have the highest chance of progressing to become invasive breast cancer. In particular, we are developing targeted nanobiosensors to test real-time protein...

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Bibliographic Details
Main Authors: Vo-Dinh, Tuan, Seewaldt, Victoria, Zhang, Yan, Scaffidi, Jonathan P, Gregas, Molly K, Lauly, Benoit, De Vience, Stephen, Dhawan, Anuj, Wang, Hsinneng, Yuan, Hsiang-Kuo
Format: Report
Language:English
Subjects:
BREAST CANCER
CELLS(BIOLOGY)
DETECTION
DYNAMIC TESTS
ELECTRON MICROSCOPY
FIBER OPTICS
Fiber Optics and Integrated Optics
FIBER-OPTIC NANOPROBE
LIFE(BIOLOGY)
Medicine and Medical Research
MICROSCOPY
OPTICAL ANALYSIS
OPTICAL DETECTION
OPTICAL SCANNING
PHOSPHORYLATION
PROTEINS
RESOLUTION
RISK
SCANNING ELECTRON MICROSCOPES
SIGNALS
SINGLE-CELL ANALYSIS
TEST AND EVALUATION
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Summary:The goal of our study is to develop nanosensors to detect pre-cancerous changes in the breast and figure out which pre-cancerous breast changes have the highest chance of progressing to become invasive breast cancer. In particular, we are developing targeted nanobiosensors to test real-time protein phosphorylation signaling in live RPFNA cells. In this study, we have designed fiber-optic nanoprobes for optical detection in single living cells. The nanoprobes were fabricated with well-controlled nanoapertures for optimized spatial resolution and optical transmission. The nanoprobes were characterized with scanning electron microscopy and near-field scanning optical microscopy to ensure optimal performance. Theoretical analysis determined the probing depth and volume of the nanoprobe. The nanoprobes have been demonstrated for detection of biologically significant species in single living cells. The nanobiosensors allow us to directly test for complex protein changes in breast cells taken from high-risk women. The original document contains color images.