Immune Responses and Protection of Aotus Monkeys Immunized with Irradiated Plasmodium vivax Sporozoites

A non-human primate model for the induction of protective immunity against the pre-erythrocytic stages of Plasmodium vivax malaria using radiation-attenuated P vivax sporozoites may help to characterize protective immune mechanisms and identify novel malaria vaccine candidates. Immune responses and...

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Bibliographic Details
Main Authors: Jordan-Villegas, Alejandro, Bonelo Perdomo, Anilza, Epstein, Judith E, Lopez, Jesus, Castellanos, Alejandro, Manzano, Maria R, Hernandez, Miguel A, Soto, Liliana, Mendez, Fabian, Richie, Thomas L
Format: Report
Language:English
Subjects:
AOTUS MONKEY
ERYTHROCYTES
GLANDS
GROUP DYNAMICS
IMMUNITY
IMMUNIZATION
INDUCTION SYSTEMS
INOCULATION
IRRADIATION
MALARIA
Medicine and Medical Research
MONKEYS
PLASMODIUM VIVAX
PRIMATES
PROTECTION
REPRINTS
RESPONSE(BIOLOGY)
SALIVA
SALIVARY GLANDS
SPOROZOITES
VACCINES
VEINS
VIABILITY
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Summary:A non-human primate model for the induction of protective immunity against the pre-erythrocytic stages of Plasmodium vivax malaria using radiation-attenuated P vivax sporozoites may help to characterize protective immune mechanisms and identify novel malaria vaccine candidates. Immune responses and protective efficacy induced by vaccination with irradiated P vivax sporozoites were evaluated in malaria-naive Aotus monkeys. Three groups of six monkeys received two, five, or ten intravenous inoculations, respectively, of 100,000 irradiated P. vivax sporozoites; control groups received either 10 doses of uninfected salivary gland extract or no inoculations. Immunization resulted in the production low levels of antibodies that specifically recognized P. vivax sporozoites and the circumsporozoite protein. Additionally immunization induced low levels of antigen-specific IFN-y responses. Intravenous challenge with viable sporozoites resulted in partial protection in a dose-dependent manner.l11ese findings suggest that the Aotus monkey model may be able to play a role in preclinical development of P. vivax pre-erythrocytic stage vaccines. Published in in American Journal of Tropical Medicine and Hygiene, v84 (Suppl 2) p43-50, 2011.