Volunteer Challenge With Enterotoxigenic Escherichia coli That Express Intestinal Colonization Factor Fimbriae CS17 and CS19

Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CSI7 and CSI9. LSN03-016011/A (LT, CS17) elicited a dosedependent eff...

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Bibliographic Details
Main Authors: McKenzie, Robin, Porter, Chad K, Cantrell, Joyce A, DeNearing :Aisling, Barbara, Grahek, Shannon L, Sincock, Stephanie A, Woods, Colleen, Sebeny, Peter, Sack, David A
Format: Report
Language:English
Subjects:
COLONIES(BIOLOGY)
DOSAGE
ENTEROTOXINS
ESCHERICHIA COLI
FIMBRIAE
INTESTINES
Medicine and Medical Research
Microbiology
PATHOGENESIS
RESPONSE(BIOLOGY)
STRAINS(BIOLOGY)
VOLUNTEERS
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Summary:Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CSI7 and CSI9. LSN03-016011/A (LT, CS17) elicited a dosedependent effect, with the upper dose (6 X 10(exp 9) organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CSI9) also showed a dose response, with a 44% diarrhea rate at 9 X 10(exp 9 organisms. Both strains elicited homologous antifimbrial and anti-L T antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT!CS17-ETEC challenge model for interventional trials. Pub. in the Journal of Infectious Disease, v204, p60-64, 2011.