Effects of Repeated Exposure to Filtered and Unfiltered Broadband Light Radiation on Escherichia coli Growth and Propagation

There is a high probability biological organisms will become inactive after exposure to non-ionizing radiation. Although continuous wave, low-pressure Hg lamps that emit UV-C radiation are recognized as effective for inactivating various microorganisms, there are other light sources that may be as e...

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Bibliographic Details
Main Authors: Wick, Charles H, Wade, Mary M, Biggs, Tracey D, Williams, Leslie I, Zulich, Alan W, Wengraitis, Stephen P, McCubbin, Patrick E
Format: Report
Language:English
Subjects:
BIOLOGICAL AGENTS
BROADBAND
BROADBAND SPECTRUM
Chemical, Biological and Radiological Warfare
CONTINUOUS WAVES
DECONTAMINATION
ESCHERICHIA COLI
NON-IONIZING RADIATION
Optics
PULSED LAMP
ULTRAVIOLET RADIATION
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Summary:There is a high probability biological organisms will become inactive after exposure to non-ionizing radiation. Although continuous wave, low-pressure Hg lamps that emit UV-C radiation are recognized as effective for inactivating various microorganisms, there are other light sources that may be as effective. The objective of the experiments conducted during this study was to measure the inactivation efficiency of pulsed non-ionizing radiation on Escherichia coli using a broadband light system, broadband bandpass filters, and (in the UV range) narrow bandpass filters. The effectiveness of pulsed UV-C radiation was also compared to that from a continuous wave, low-pressure Hg light system. E. coli was plated onto tryptic soy agar and exposed to the filtered and unfiltered light sources. After exposure, all plates were incubated and then examined for growth or lack thereof to determine the inactivation effectiveness of broadband light and continuous wave light systems on E. coli. Prepared in Optimetrics, Inc., Abingdon, MD. The original document contains color images.