Acute Inhalation Toxicity and Blood Absorption of 2,4-Dinitroanisole (DNAN) in Rats

This toxicology study was conducted to determine the 4 -hour inhalation median lethal concentration (LC50) of 2,4-Dinitroanisole (DNAN) in male and female rats . Nose-only exposure to the highest-achievable aerosol atmosphere of DNAN (2,4 mg/L) did not induce any compound-related mortality, adverse...

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Bibliographic Details
Main Authors: Crouse, Lee C, O'Neill, Arthur J
Format: Report
Language:English
Subjects:
ABSORPTION
ACUTE INHALATION TOXICITY
Anatomy and Physiology
ANISOLE
Biochemistry
BLOOD ABSORPTION STUDIES
BLOOD CHEMISTRY
BLOOD SAMPLES
BODY WEIGHT
CONCENTRATION(CHEMISTRY)
DNAN(2 4-DINITROANISOLE)
DNP(2 4-DINITROPHENOL)
EXPOSURE(PHYSIOLOGY)
INHALATION
LC50
LETHAL DOSAGE
MORTALITY RATE
ORAL GAVAGE
Organic Chemistry
PATHOLOGY
RATS
ROUTES OF ADMINISTRATION
SAMPLING
STATISTICAL ANALYSIS
TOXICITY
Toxicology
URINE
URINE SAMPLES
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Summary:This toxicology study was conducted to determine the 4 -hour inhalation median lethal concentration (LC50) of 2,4-Dinitroanisole (DNAN) in male and female rats . Nose-only exposure to the highest-achievable aerosol atmosphere of DNAN (2,4 mg/L) did not induce any compound-related mortality, adverse toxic signs, body weight changes, or gross necropsy findings. A secondary objective was to determine the effect that two different routes of administration (inhalation and oral) had on the absorption of the chemical into the bloodstream . Blood samples were collected and analyzed from exposed rats at 7 time points for those exposed via inhalation and 6 different time points for those given a calculated equivalent oral dose. The results of the blood absorption study indicated that, under the stated study conditions and limitations, acute exposure to DNAN via oral gavage appears to induce higher DNAN whole blood concentrations in laboratory rats compared to those exposed via inhalation. Blood concentrations of the metabolite 2,4-Dinitrophenol (DNP) were not different between the two exposure routes.