Rapid Field-Usable Cyanide Sensor Development for Blood and Saliva

• Main Authors: Logue, Brian A, Stutelberg, Michael W, Manandhar, Erica, Dzisam, Joseph K, Mitchell, Brendan L, Jackson, Randy E, Zhou, Wenhui, Oda, Robert P, Bhandari, Raj K
• Format: Report
• Language: English
• Subjects: 2-AMINO-2-THIAZOLINO-4-CA RBOXYL IC ACID; BIOMARKER; CHEMICAL REACTIONS; CYANIDES; DETECTORS; DIAGNOSTIC EQUIPMENT; DMTS; EXPOSURE(PHYSIOLOGY); IDENTIFICATION; Inorganic Chemistry; Medicine and Medical Research; POISONS; SULFANEGEN. COBINAMIDE; THERAPEUTICS; THERAPY; THIOCYANATE; TOXICOKINETICS

Cyanide is a deadly poison which may be ingested or inhaled and can cause severe incapacitation or death. The diagnosis of cyanide exposure is critical to speed treatment and reduce harm. The development of a diagnostic sensor device and the identification and analysis of novel biomarkers of cyanide exposure and the development of methods of analysis for novel cyanide therapeutics are the major objectives of this research. Since the onset of toxic outcome from cyanide exposure is very fast, a rapid and portable sensor for the detection of cyanide exposure was developed and tested. The sensor utilized a cyanide-selective fluorescent reaction as the core chemical reaction with micro-diffusion sample preparation and can determine exposure 60s. Multiple novel markers of cyanide exposure were also identified as having potential advantages to cyanide and thiocyanate, and methods of analysis for these markers were developed or are in the process of being developed. Specifically, 2-amino-2-thiozoline-4-carboxylic acid (ATCA), alpha-ketoglutarate, and a cyanide-glutathione adduct were investigated and obtained. Methods of analysis for DMTS, Cbi, and sulfanegen were developed and utilized. The original document contains color images.