Metal Ion Sensor with Catalytic DNA in a Nanofluidic Intelligent Processor

This paper presents the potential of combining Pb2+ specific catalytic DNA enzymes with microfluidic devices. We incorporate the catalytic DNAzyme into a detection channel on the microfluidic device. Lead containing solution is placed in a source channel. By appropriate application of voltages acros...

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Bibliographic Details
Main Authors: Cropek, Donald M, Bohn :In-Hyoung, Paul W, Sweedler, Jonathan, Lu, Yi, Swearingen, Carla, Wernette, Daryl
Format: Report
Language:English
Subjects:
CATALYSIS
CATALYTIC DNA
DEOXYRIBONUCLEIC ACIDS
DETECTORS
FLUIDICS
Industrial Chemistry and Chemical Processing
IONS
LEAD COMPOUNDS
METAL ION SENSORS
MICROFLUIDIC DEVICES
Miscellaneous Detection and Detectors
NANOTECHNOLOGY
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Summary:This paper presents the potential of combining Pb2+ specific catalytic DNA enzymes with microfluidic devices. We incorporate the catalytic DNAzyme into a detection channel on the microfluidic device. Lead containing solution is placed in a source channel. By appropriate application of voltages across channels, the lead solution can be injected through nanocapillary array interconnects (NAI) that separate the channels. These gatable microfluidic devices build upon research developed by Bohn and Sweedler et al. (16-18). Methods for adapting lead specific biosensors to the NAI microfluidic device are presented. Performances of the developed method were evaluated by studying quantifiable concentration range, method accuracy and precision. Successful application to real sample analysis using an electroplating sludge certified reference material is also presented. Prepared in collaboration with the University of Illinois, Urbana-Champaign. The original document contains color images.