Nosocomial dissemination of plasmids carrying blaTEM-24 , blaDHA-1 , aac ( 6 ′)- Ib - cr , and qnr A6 in Providencia spp. strains isolated from a Tunisian hospital

Abstract The aim of this study is to report the emergence of IncA/C conjugative plasmids harboring blaTEM-24 , blaDHA-1 , qnr A6, and aac ( 6 ′)- Ib - cr genes among Providencia spp. isolates recovered in 2008 in Tunisia. The double-disk synergy test confirmed the phenotype extended-spectrum β-lacta...

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Published in:Diagnostic microbiology and infectious disease 2015, Vol.81 (1), p.50-52
Main Authors: Mahrouki, Sihem, Chihi, Hela, Bourouis, Amel, Ayari, Khaoula, Ferjani, Mustapha, Moussa, Mohamed Ben, Belhadj, Omrane
Format: Article
Language:English
Subjects:
Infectious Disease
Internal Medicine
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Summary:Abstract The aim of this study is to report the emergence of IncA/C conjugative plasmids harboring blaTEM-24 , blaDHA-1 , qnr A6, and aac ( 6 ′)- Ib - cr genes among Providencia spp. isolates recovered in 2008 in Tunisia. The double-disk synergy test confirmed the phenotype extended-spectrum β-lactamase (ESBL) in 2 Providencia stuartii and 5 Providencia rettgeri . These ESBLs were coresistant to cefoxitin, chloramphenicol, ofloxacin, and sulfonamides but remained susceptible to imipenem. Three β-lactamases TEM-2, TEM-24, and DHA-1 were detected. blaTEM-24 , blaDHA-1 , aac ( 6 ′)- Ib - cr , and qnr A6 genes were successfully transferred to Escherichia coli strain HB101, and they were found located on the conjugatifs IncA/C plasmids. Genetic relatedness showed similar and different patterns among P. stuartii and P. rettgeri strains, respectively.
ISSN:0732-8893
DOI:10.1016/j.diagmicrobio.2014.09.021