Structural and functional abnormalities of hepatic tissues of male Wistar rats fed on hyper whey and super amino anabolic protein

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2016
• Main Authors: Ali, Doaa A, El-Sayyad, Hassan IH, Moftah, Osama A, Chilibeck, Philip D
• Format: Article
• Language: English
• Subjects: Gastroenterology and Hepatology
• ISSN: 0899-9007
• DOI: 10.1016/j.nut.2016.01.017

Abstract Objective Athletes and bodybuilders consumed high protein supplements to obtain energy and enhance the development and strength of their muscles. Over time different investigations have revealed dysfunctions of their body organs. There are contradictions between scientists concerning the benefits and the alarm of developing body dysfunction. Methods We assigned male Wistar albino rats to 3 groups(n=10 each), control, hyperwhey protein (Protein formula, Nutrabolics, Richmond,Canada) (2.5g/kg body weight) and super amino 2500 (SA) ( Launderale ,FL 33301, USA). (2.5g/kg body weight). The applied dose was orally administered daily in tap water for 14 weeks. Body weight was regularly measured. At 14 weeks, animals were sacrificed and dissected. Blood was collected from a puncture of heart and liver was removed and weighed. Biochemical analysis of liver function tests, lipidogram, haematology, histopathology, transmission electron microscopy, immunohistochemistry of proliferating cell nuclear antigen (PCNA), B-cell lymphoma 2 (Bcl2) and 70 kilodalton heat shock proteins (HS70) and flow-cytometry of hepatocyte cell cycle were performed Results Hyperwhey and super amino (SA) supplemented rats had lower body weight gain in comparison with the control and developed hepatic dysfunction manifested by apparent congestion of blood vessel, increased apoptosis and breakdown of hepatocytes. SA had thickening of the liver capsule and more drastic damage of hepatocytes. The level of transaminases was markedly increased. Also insulin level was markedly decreased in parallel with increase cholesterol, LDL and triglycerides. Conclusion Hyperwhey and super amino protein formula administration dramatically altered the liver function and increased hepatic damage similar to the development of suspected diabetes.