Biodistribution, pharmacokinetics and imaging of188 Re-BMEDA-labeled pegylated liposomes after intraperitoneal injection in a C26 colon carcinoma ascites mouse model

• Published in: Nuclear medicine and biology 2007, Vol.34 (4), p.415-423
• Main Authors: Chen, Liang-Cheng, Chang, Chih-Hsien, Yu, Chia-Yu, Chang, Ya-Jane, Hsu, Wei-Chuan, Ho, Chung-Li, Yeh, Chung-Hsin, Luo, Tsai-Yueh, Lee, Te-Wei, Ting, Gann
• Format: Article
• Language: English
• Subjects: Radiology
• ISSN: 0969-8051
• DOI: 10.1016/j.nucmedbio.2007.02.003

Abstract Nanoliposomes are important carriers capable of packaging drugs for various delivery applications through passive targeting tumor sites by enhanced permeability and retention effect. Radiolabeled liposomes have potential applications in radiotherapy and diagnostic imaging. The purpose of this study was to investigate the biodistribution, pharmacokinetics and imaging of nanotargeted188 Re- N , N -bis (2-mercaptoethyl)- N ′, N ′-diethylethylenediamine (BMEDA)-labeled pegylated liposomes (RBLPL) and unencapsulated188 Re-BMEDA after intraperitoneal (ip) injection in a C26 colon carcinoma ascites mouse model. The nanopegylated liposomes were labeled with188 Re-BMEDA. The labeling efficiency of RBLPL was 82.3±4.5%. In vitro stability of RBLPL in normal saline at room temperature and in rat plasma at 37°C for 72 h was 92.01±1.31% and 82.4±1.64%, respectively. The biodistribution studies indicated that the radioactivity in ascites was 69.96±14.08 percentage injected dose per gram (% ID/g) at 1h to 5.99±1.97% ID/g at 48 h after ip administration of RBLPL. The levels of radioactivity in tumor were progressive accumulation to a maximum of 6.57±1.7% ID/g at 24 h. The radioactivity of188 Re-BMEDA in ascites reached the maximum level of 54.89±5.91% ID/g at 1 h and declined rapidly with time. Pharmacokinetic studies revealed that the terminal half-life, total body clearance and area under the curve of RBLPL were 5.3-, 9.5- and 9.4-fold higher than that of188 Re-BMEDA in blood, respectively. These results suggested that the long circulation, bioavailability and localization of RBLPL in tumor and ascites sites, which also demonstrate that the ip administration of RBLPL is a potential multifunctional nanoradiotherapeutics and imaging agents on a C26 colon carcinoma ascites mouse model.