Exercise training contributes to H2 O2 /VEGF signaling in the lung of rats with monocrotaline-induced pulmonary hypertension

• Published in: Vascular pharmacology 2016
• Main Authors: Colombo, Rafael, Siqueira, Rafaela, Conzatti, Adriana, de Lima, Bruna Gazzi, Fernandes, Tânia Regina Gattelli, Godoy, Alessandra Eifler Guerra, Litvin, Isnard Elman, Silva, Jairo Montemor Augusto, Tucci, Paulo José Ferreira, da Rosa Araújo, Alex Sander, Belló-Klein, Adriane
• Format: Article
• Language: English
• Subjects: Cardiovascular
• ISSN: 1537-1891
• DOI: 10.1016/j.vph.2016.06.006

Abstract Pulmonary arterial hypertension (PAH) is characterized by pulmonary vascular remodeling and right ventricle overload. Given that angiogenesis is a key factor involved in the reduction of vascular resistance to blood flow, we tested the hypothesis that aerobic exercise exerts a positive impact on hydrogen peroxide (H2 O2 ) and protein kinase B (Akt) levels in the lung parenchyma. To study the effects of aerobic exercise on lung angiogenesis signaling, Wistar rats were administered monocrotaline (MCT) (60 mg/kg i.p.) or the same volume of saline (0.9% NaCl i.p.). There was an increase in H2 O2 (43%) in PAH-trained animals (TM) compared to control animals (SM). H2 O2 showed a positive correlation ( r = 0.77) with vascular endothelial growth factor (VEGF). VEGF was higher (4.7 fold) in TM animals compared to SM. VEGF and angiopoietin-1 (Ang-1) showed positive staining in the lung parenchyma of TM and SM. Glutathione peroxidase showed higher activity in the TM group (49%) compared to trained control (TC). Aerobic exercise increased the activity of peroxiredoxin ( P < 0.05). The increase in VEGF was positively correlated with Akt phosphorylation ( r = 0.73). p -Akt was shown to be increased in TM animals when compared to SM animals (2.5 fold). The change in fractional area, fractional shortening and systolic tricuspid annular plane excursion showed improvement after exercise training. Therefore, aerobic exercise promotes H2 O2 /VEGF/p-Akt signaling for pulmonary physiological angiogenesis. It is associated with an improvement in RV function, as evaluated by echocardiography.