Micro Patterning of Nanocomposite Polymer Scaffolds Using Sacrificial Phosphate Glass Fibres for Tendon Tissue Engineering Applications

Abstract This study presents a simple and reproducible method of micro patterning the novel nanocomposite polymer (POSS-PCU) using a sacrificial phosphate glass fiber template for tendon tissue engineering applications. The diameters of the patterned scaffolds produced were dependent on the diameter...

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Bibliographic Details
Published in:Nanomedicine 2017
Main Authors: Alshomer, Feras, Chaves, Camilo, Serra, Tiziano, Ahmed, Ifty, Kalaskar, Deepak M
Format: Article
Language:English
Subjects:
Internal Medicine
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Summary:Abstract This study presents a simple and reproducible method of micro patterning the novel nanocomposite polymer (POSS-PCU) using a sacrificial phosphate glass fiber template for tendon tissue engineering applications. The diameters of the patterned scaffolds produced were dependent on the diameter of the glass fibers (15 μm) used. Scaffolds were tested for their physical properties and reproducibility using various microscopy techniques. For the first time, we show that POSS-PCU supports growth of human tenocytes cells. Furthermore, we show that cellular alignment, their biological function and expression of various tendon related proteins such as scleraxis, collagen I and III, tenascin-C are significantly elevated on the micro patterned polymer surfaces compared to flat samples. This study demonstrated a simple, reproducible method of micro patterning POSS-PCU nanocomposite polymer for novel tendon repair applications, which when provided with physical cues could help mimic the microenvironment of tenocytes cells.
ISSN:1549-9634
DOI:10.1016/j.nano.2017.01.006