The influence of E. coliimplantation in axenic mice on cytokine production by peritoneal and bone marrow-derived macrophages

To assess the involvement of bacterial microflora in the development of host defenses, we compared in vitro LPS-induced cytokine production by macrophages in germ-free and E. colimonoxenic mice. E. coliimplantation significantly increased IL-1 and IL-6 and, to a lesser extent, TNF activities of peri...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 1995, Vol.7 (7), p.713-719
Main Authors: Nicaise, Pascale, Gleizes, Aude, Forestier, Fran çoise, Sandre, Catherine, Quero, Anne-Marie, Labarre, Colette
Format: Article
Language:English
Subjects:
bone marrow-derived macrophages
cytokine
E. coli
germ-free
peritoneal macrophages
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Summary:To assess the involvement of bacterial microflora in the development of host defenses, we compared in vitro LPS-induced cytokine production by macrophages in germ-free and E. colimonoxenic mice. E. coliimplantation significantly increased IL-1 and IL-6 and, to a lesser extent, TNF activities of peritoneal and bone marrow-derived macrophages. These results suggest that exposure to microflora primes macrophages for an enhanced cytokine production, which may contribute to the activation of the antiinfectious defense. The priming was not restricted to peritoneal macrophages but was associated with a more general effect of the flora since the enhanced response of bone marrow-derived macrophages indicates an effect on macrophage precursors. Furthermore, a higher ability of peritoneal macrophages to produce IL-1 in axenic and monoxenic mice was observed as compared to bone marrow-derived macrophages. In contrast, bone marrow-derived macrophages demonstrated a higher ability to produce IL-6 and TNF but only 3 weeks after bacterial administration.
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.1995.0084