Control of DrosophilaOpsin Gene Expression by Carotenoids and Retinoic Acid: Northern and Western Analyses

• Published in: Experimental eye research 1996, Vol.63 (5), p.493-500
• Main Authors: PICKING, WENDY L., CHEN, DE-MAO, LEE, RONNIE D., VOGT, MATTHEW E., POLIZZI, JOSEPH L., MARIETTA, ROBERT G., STARK, WILLIAM S.
• Format: Article
• Language: English
• Subjects: mRNA; Northern analysis; Retinoic acid; Rhodopsin; transcription; vitamin A; Western blots
• ISSN: 0014-4835; 1096-0007
• DOI: 10.1006/exer.1996.0139

In the fly, thorough retinoid deprivation is possible, to optimize investigation of the effects of vitamin A metabolites and retinoic acid (RA) on visual development. Retinoids had been found to control fly opsin gene transcription, though this finding was contested. Northern blots on Drosophilaheads showed that mRNA of Rh1 (the predominant rhodopsin) was high in vitamin A replete controls, very low in deprived flies, and increased upon feeding carrot juice to deprived flies as early as 1 hr. Expression of the ribosomal protein 49 [rp49] gene (the control) was equal both in deprivation and in replacement. Recovery of Rh1 protein upon such carotenoid replacement followed, barely detectable on Western blots at 4 hr but conspicuous by 8 hr. Alternative chromophore deprivation with yeast–glucose food yielded flies with opsin mRNA on Northerns but not rhodopsin, as demonstrated by Western blots, spectrophotometry and the electroretinogram (ERG). Rh1's mRNA but not Rh1 protein resulted from rearing flies from egg to adult on the otherwise deprivational medium supplemented with RA or beef brain–heart infusion. By comparing results from these different media it was concluded that: [1] deprivation and replacement affect opsin gene transcription; and [2] contradictory conclusions were from chromophore deprivation which does not eliminate all retinoid dependent factors which could affect the opsin promoter. Preliminary evidence shows that carotenoid deprivation decreases two proteins relevant to visual function: [1] phospholipase C (PLC); and [2] Drosophilaretinoid binding protein (DRBP).