Accelerated development of genital Chlamydia trachomatisserovar E in McCoy cells grown on microcarrier beads

• Published in: Microbial pathogenesis 1996, Vol.20 (1), p.31-40
• Main Authors: Wyrick, Priscilla B., Gerbig Jr, Donald G., Knight, Stephen T., Raulston, Jane E.
• Format: Article
• Language: English
• Subjects: accelerated development cycle; Chlamydia trachomatis; infectivity titration; microcarrier beads; particle count
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1006/mpat.1996.0003

Chlamydia trachomatisserovar E is a major cause of bacterially-acquired sexually transmitted infections. Stock cultures of these obligate intracellular bacteria are often propogated in McCoy cells. We recently reported that greater infectious titers of chlamydiae could be obtained if the McCoy cells were cultured on collagen-coated microcarrier beads versus plastic flasks, although the reason for the difference in efficiency was not clear. This study analyzed the development of C. trachomatisgrown in McCoy cells by the two methods. Transmission electron microscopy analysis revealed an accelerated chlamydial development, with maturation of reticulate bodies into elementary bodies sooner in McCoy cells grown on the porous substratum. Comparison of particle counts versus infectivity titers indicated the production of fewer numbers of elementary bodies but which were highly infectious sooner from the infected McCoy cell-microcarrier bead cultures than from duplicate infected McCoy cell cultures grown in plastic tissue culture flasks.