REPEATED OVARIAN STIMULATION ALTERS TELOMERE LENGTH IN MOUSE OVARIAN FOLLICLES

Assisted reproductive technologies (ARTs) are commonly used to treat infertility. One of the fundamental steps in ART is the controlled ovarian stimulation, also known as superovulation. Whereas superovulation increases the number of mature oocytes, it can lead to various adverse effects, such as in...

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Bibliographic Details
Published in:Reproductive biomedicine online 2024-11, Vol.49
Main Authors: TIRE, Betül, OZTURK, Saffet
Format: Article
Language:English
Subjects:
ovarian follicles
Q-FISH
Repeated ovarian stimulation
telomere length
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Summary:Assisted reproductive technologies (ARTs) are commonly used to treat infertility. One of the fundamental steps in ART is the controlled ovarian stimulation, also known as superovulation. Whereas superovulation increases the number of mature oocytes, it can lead to various adverse effects, such as increased oxidative stress, decreased oocyte and embryo quality, and mitochondrial dysfunction. The adverse effects of repeated superovulation largely overlap with the phenotypes occurring due telomere length changes. This study aimed to evaluate the impact of repeated superovulation on telomere length in mouse ovaries. Four groups from Balb/C female mice in the diestrus phase of the estrus cycle were created as follows: Control (C;n=6 for germinal vesicle (GV) and n=6 for metaphase II (MII) oocyte collection), once superovulated (1S;n=6 for GV and n=6 for MII oocyte collection), three times superovulated (3S; n=6 for GV and n=6 for MII oocyte collection), and five times superovulated (5S;n=6 for GV and n=6 for MII oocyte collection) with 1-week intervals. The GV oocytes were obtained 44 hours after intraperitoneal injection of 8 IU pregnant mare's serum gonadotropin (PMSG). MII oocytes were collected from oviducts of the females 14 hours after injection with 8 IU human chorionic gonadotropin (hCG), which had been primed with 8 IU PMSG 48 hours before. Control mice were injected with sterile water five times before obtaining GV and MII oocytes. For MII oocyte collection in the control group, female mice were mated with vasectomized males. After the respective treatments, ovaries were collected from each group for telomere length analysis. Telomere length in the ovaries and their follicles was determined using quantitative fluorescence in situ hybridization (Q-FISH). Data were analyzed with ImageJ; P
ISSN:1472-6483
DOI:10.1016/j.rbmo.2024.104583