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Biocolorant “prodigiosin” interferes with the growth of biofouling bacteria: an in vitro and in silico approach
Purpose The purpose of this paper was to identify Serratia marcescens to extract and purify prodigiosin pigment to evaluate the antibacterial potential of the pigment. Design/methodology/approach Samples were collected from shrimp aquaculture ponds. Species identification was conducted using morphol...
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| Published in: | Pigment & resin technology 2022-01, Vol.51 (1), p.24-32 |
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| Main Authors: | , , , |
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| cites | cdi_FETCH-LOGICAL-c311t-57d61c1ef7cf91be421203a2d75b91c77b144bdbc745491cf436c04f4341f06b3 |
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| container_title | Pigment & resin technology |
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| creator | Mousavi, Seyedeh Maryam Archangi, Bita Zolgharnein, Hosein Zamani, Isaac |
| description | Purpose
The purpose of this paper was to identify Serratia marcescens to extract and purify prodigiosin pigment to evaluate the antibacterial potential of the pigment.
Design/methodology/approach
Samples were collected from shrimp aquaculture ponds. Species identification was conducted using morphological, biochemical and molecular tests. Pigment extraction and purification were carried out using column chromatography. The antibacterial effect of crude and purified prodigiosin pigment was evaluated on Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus as biofouling bacteria. In addition, the interaction between prodigiosin and proteins involved in biofilm formation was evaluated using molecular docking.
Findings
The results of prodigiosin extraction with solvents showed the highest percentage of pigment presence with methanol solvent in the second day of culture. The chemical structure of pure prodigiosin obtained from the column chromatography was confirmed by Fourier-transform infrared spectroscopy. Both crude and purified pigments exhibited antibacterial effects against selected bacterial strains. The antibacterial effect of the purified pigment was higher, and the highest antibacterial effect was observed on B. subtilis. Prodigiosin docking was carried out with all target proteins, and the docked energy in all of them was at an acceptable level.
Originality/value
Prodigiosin extracted from S. marcescens can be used as a bioactive compound to design and manufacture of anti-biofouling and anti-biofilm formation products to use extensively for industrial applications as a natural color in marine industries, food industry, cosmetics and textile productions. |
| doi_str_mv | 10.1108/PRT-07-2020-0079 |
| format | article |
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The purpose of this paper was to identify Serratia marcescens to extract and purify prodigiosin pigment to evaluate the antibacterial potential of the pigment.
Design/methodology/approach
Samples were collected from shrimp aquaculture ponds. Species identification was conducted using morphological, biochemical and molecular tests. Pigment extraction and purification were carried out using column chromatography. The antibacterial effect of crude and purified prodigiosin pigment was evaluated on Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus as biofouling bacteria. In addition, the interaction between prodigiosin and proteins involved in biofilm formation was evaluated using molecular docking.
Findings
The results of prodigiosin extraction with solvents showed the highest percentage of pigment presence with methanol solvent in the second day of culture. The chemical structure of pure prodigiosin obtained from the column chromatography was confirmed by Fourier-transform infrared spectroscopy. Both crude and purified pigments exhibited antibacterial effects against selected bacterial strains. The antibacterial effect of the purified pigment was higher, and the highest antibacterial effect was observed on B. subtilis. Prodigiosin docking was carried out with all target proteins, and the docked energy in all of them was at an acceptable level.
Originality/value
Prodigiosin extracted from S. marcescens can be used as a bioactive compound to design and manufacture of anti-biofouling and anti-biofilm formation products to use extensively for industrial applications as a natural color in marine industries, food industry, cosmetics and textile productions.</description><identifier>ISSN: 0369-9420</identifier><identifier>EISSN: 1758-6941</identifier><identifier>DOI: 10.1108/PRT-07-2020-0079</identifier><language>eng</language><publisher>Bradford: Emerald Publishing Limited</publisher><subject>Antiinfectives and antibacterials ; Aquaculture ; Bacteria ; Biofilms ; Biofouling ; Column chromatography ; Columnar structure ; Cosmetics ; E coli ; Fourier transforms ; Glycerol ; Industrial applications ; Microorganisms ; Phenols ; Pigments ; Proteins ; Pseudomonas aeruginosa ; Solvents</subject><ispartof>Pigment & resin technology, 2022-01, Vol.51 (1), p.24-32</ispartof><rights>Emerald Publishing Limited</rights><rights>Emerald Publishing Limited.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-57d61c1ef7cf91be421203a2d75b91c77b144bdbc745491cf436c04f4341f06b3</citedby><cites>FETCH-LOGICAL-c311t-57d61c1ef7cf91be421203a2d75b91c77b144bdbc745491cf436c04f4341f06b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Mousavi, Seyedeh Maryam</creatorcontrib><creatorcontrib>Archangi, Bita</creatorcontrib><creatorcontrib>Zolgharnein, Hosein</creatorcontrib><creatorcontrib>Zamani, Isaac</creatorcontrib><title>Biocolorant “prodigiosin” interferes with the growth of biofouling bacteria: an in vitro and in silico approach</title><title>Pigment & resin technology</title><description>Purpose
The purpose of this paper was to identify Serratia marcescens to extract and purify prodigiosin pigment to evaluate the antibacterial potential of the pigment.
Design/methodology/approach
Samples were collected from shrimp aquaculture ponds. Species identification was conducted using morphological, biochemical and molecular tests. Pigment extraction and purification were carried out using column chromatography. The antibacterial effect of crude and purified prodigiosin pigment was evaluated on Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus as biofouling bacteria. In addition, the interaction between prodigiosin and proteins involved in biofilm formation was evaluated using molecular docking.
Findings
The results of prodigiosin extraction with solvents showed the highest percentage of pigment presence with methanol solvent in the second day of culture. The chemical structure of pure prodigiosin obtained from the column chromatography was confirmed by Fourier-transform infrared spectroscopy. Both crude and purified pigments exhibited antibacterial effects against selected bacterial strains. The antibacterial effect of the purified pigment was higher, and the highest antibacterial effect was observed on B. subtilis. Prodigiosin docking was carried out with all target proteins, and the docked energy in all of them was at an acceptable level.
Originality/value
Prodigiosin extracted from S. marcescens can be used as a bioactive compound to design and manufacture of anti-biofouling and anti-biofilm formation products to use extensively for industrial applications as a natural color in marine industries, food industry, cosmetics and textile productions.</description><subject>Antiinfectives and antibacterials</subject><subject>Aquaculture</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Biofouling</subject><subject>Column chromatography</subject><subject>Columnar structure</subject><subject>Cosmetics</subject><subject>E coli</subject><subject>Fourier transforms</subject><subject>Glycerol</subject><subject>Industrial applications</subject><subject>Microorganisms</subject><subject>Phenols</subject><subject>Pigments</subject><subject>Proteins</subject><subject>Pseudomonas aeruginosa</subject><subject>Solvents</subject><issn>0369-9420</issn><issn>1758-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNptUctKAzEUDaJgre5dBlyPvclkJo07FV9QUKSuQyaTtCnTSU2mFnf9EP25fokZ6kZwc1-cB5yL0DmBS0JgPHp5nWbAMwoUMgAuDtCA8GKclYKRQzSAvBSZYBSO0UmMCwCglIsBijfOa9_4oNoO77Zfq-BrN3M-una3_cau7UywJpiIN66b425u8Cz4TRq9xZXz1q8b185wpXRCOnWFVZtY-MN1wae57pfoGqfTtkrqSs9P0ZFVTTRnv32I3u7vpreP2eT54en2epLpnJAuK3hdEk2M5doKUhlGCYVc0ZoXlSCa84owVtWV5qxg6WBZXmpgqTFioazyIbrY6ybb97WJnVz4dWiTpaQloYUQqSQU7FE6-BiDsXIV3FKFT0lA9tHKFK0ELvtoZR9tooz2FLM0QTX1f4w_z8h_AMWrfVI</recordid><startdate>20220103</startdate><enddate>20220103</enddate><creator>Mousavi, Seyedeh Maryam</creator><creator>Archangi, Bita</creator><creator>Zolgharnein, Hosein</creator><creator>Zamani, Isaac</creator><general>Emerald Publishing Limited</general><general>Emerald Group Publishing Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7WY</scope><scope>7XB</scope><scope>8AF</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>K6~</scope><scope>KB.</scope><scope>L.-</scope><scope>M0F</scope><scope>M2P</scope><scope>PDBOC</scope><scope>PQBIZ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20220103</creationdate><title>Biocolorant “prodigiosin” interferes with the growth of biofouling bacteria: an in vitro and in silico approach</title><author>Mousavi, Seyedeh Maryam ; Archangi, Bita ; Zolgharnein, Hosein ; Zamani, Isaac</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-57d61c1ef7cf91be421203a2d75b91c77b144bdbc745491cf436c04f4341f06b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Aquaculture</topic><topic>Bacteria</topic><topic>Biofilms</topic><topic>Biofouling</topic><topic>Column chromatography</topic><topic>Columnar structure</topic><topic>Cosmetics</topic><topic>E coli</topic><topic>Fourier transforms</topic><topic>Glycerol</topic><topic>Industrial applications</topic><topic>Microorganisms</topic><topic>Phenols</topic><topic>Pigments</topic><topic>Proteins</topic><topic>Pseudomonas aeruginosa</topic><topic>Solvents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mousavi, Seyedeh Maryam</creatorcontrib><creatorcontrib>Archangi, Bita</creatorcontrib><creatorcontrib>Zolgharnein, Hosein</creatorcontrib><creatorcontrib>Zamani, Isaac</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>STEM Database</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Business Collection</collection><collection>Materials Science Database</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Trade & Industry</collection><collection>Science Database (ProQuest)</collection><collection>Materials Science Collection</collection><collection>One Business</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Pigment & resin technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mousavi, Seyedeh Maryam</au><au>Archangi, Bita</au><au>Zolgharnein, Hosein</au><au>Zamani, Isaac</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biocolorant “prodigiosin” interferes with the growth of biofouling bacteria: an in vitro and in silico approach</atitle><jtitle>Pigment & resin technology</jtitle><date>2022-01-03</date><risdate>2022</risdate><volume>51</volume><issue>1</issue><spage>24</spage><epage>32</epage><pages>24-32</pages><issn>0369-9420</issn><eissn>1758-6941</eissn><abstract>Purpose
The purpose of this paper was to identify Serratia marcescens to extract and purify prodigiosin pigment to evaluate the antibacterial potential of the pigment.
Design/methodology/approach
Samples were collected from shrimp aquaculture ponds. Species identification was conducted using morphological, biochemical and molecular tests. Pigment extraction and purification were carried out using column chromatography. The antibacterial effect of crude and purified prodigiosin pigment was evaluated on Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus as biofouling bacteria. In addition, the interaction between prodigiosin and proteins involved in biofilm formation was evaluated using molecular docking.
Findings
The results of prodigiosin extraction with solvents showed the highest percentage of pigment presence with methanol solvent in the second day of culture. The chemical structure of pure prodigiosin obtained from the column chromatography was confirmed by Fourier-transform infrared spectroscopy. Both crude and purified pigments exhibited antibacterial effects against selected bacterial strains. The antibacterial effect of the purified pigment was higher, and the highest antibacterial effect was observed on B. subtilis. Prodigiosin docking was carried out with all target proteins, and the docked energy in all of them was at an acceptable level.
Originality/value
Prodigiosin extracted from S. marcescens can be used as a bioactive compound to design and manufacture of anti-biofouling and anti-biofilm formation products to use extensively for industrial applications as a natural color in marine industries, food industry, cosmetics and textile productions.</abstract><cop>Bradford</cop><pub>Emerald Publishing Limited</pub><doi>10.1108/PRT-07-2020-0079</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0369-9420 |
| ispartof | Pigment & resin technology, 2022-01, Vol.51 (1), p.24-32 |
| issn | 0369-9420 1758-6941 |
| language | eng |
| recordid | cdi_emerald_primary_10_1108_PRT-07-2020-0079 |
| source | Emerald:Jisc Collections:Emerald Subject Collections HE and FE 2024-2026:Emerald Premier (reading list) |
| subjects | Antiinfectives and antibacterials Aquaculture Bacteria Biofilms Biofouling Column chromatography Columnar structure Cosmetics E coli Fourier transforms Glycerol Industrial applications Microorganisms Phenols Pigments Proteins Pseudomonas aeruginosa Solvents |
| title | Biocolorant “prodigiosin” interferes with the growth of biofouling bacteria: an in vitro and in silico approach |
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