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Fas (CD95) Expression is Up-Regulated on Papillary Thyroid Carcinoma1
Thyrocyte apoptosis signaled through the Fas receptor has been proposed as a mechanism for the cytotoxicity observed in thyroiditis, but the role the Fas pathway plays in thyroid cancer is not known. We examined Fas expression in thyroid tissue derived from patients with papillary carcinoma and foll...
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Published in: | The journal of clinical endocrinology and metabolism 1999-11, Vol.84 (11), p.4246-4252 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | Thyrocyte apoptosis signaled through the Fas receptor has been proposed
as a mechanism for the cytotoxicity observed in thyroiditis, but the
role the Fas pathway plays in thyroid cancer is not known. We examined
Fas expression in thyroid tissue derived from patients with papillary
carcinoma and follicular cancer. More intense immunohistological
staining for the Fas protein was observed on papillary cancer cells as
compared with adjacent normal follicles. To further characterize the
expression of Fas in papillary cancer, paired normal and cancerous
thyroid tissues were obtained at thyroidectomy from several donors,
digested, and placed into cell culture. Messenger RNA was analyzed by
ribonuclease protection assays, and protein was identified by flow
cytometry. Fas expression was detected at levels up to 3-fold higher in
cancerous thyrocytes compared with paired normal cells. To determine
whether the expressed Fas antigen was functional, thyrocytes were
treated with a monoclonal IgM anti-Fas antibody (clone CH11;
Upstate Biotechnology, Inc., Lake Placid, NY) in
the presence of interferon-γ and cycloheximide. Whereas both normal
and cancerous thyrocytes were induced to die after this treatment, the
cancerous thyrocytes were more sensitive to anti-Fas antibody. This
work demonstrates that the Fas antigen is expressed and functional on
papillary thyroid cancer cells and this may have potential therapeutic
significance. |
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ISSN: | 0021-972X 1945-7197 |
DOI: | 10.1210/jcem.84.11.6139 |