Comparison of a commercial enzyme immune assay (ELISA) and serum neutralization techniques for the detection of antibodies against BHV-1 in buffaloes (Bubalus bubalis) sera

The performance of a commercial ELISA (Bovine Rhinotracheitis virus antibody test Kit, Herdcheck®, IDEXX Laboratories Inc., USA) designed to detect the presence of antibody to the Bovine Herpesvirus type 1 (BHV-1) in bovine serum, was evaluated in samples of buffaloes (Bubalus bubalis) serum. Sera o...

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Bibliographic Details
Published in:Brazilian journal of veterinary research and animal science 2001-01, Vol.38 (3), p.146-148
Main Authors: Adriana Cortez, Marcos Bryan Heinemann, Amauri Alcindo Alfieri, Kerly Cristina Médici, Alice Fernandes Alfieri, Daniela Bergamin Oliveira, Adriana Dresden Meyer, Rodrigo Martins Soares, Sidnei Mioshi Sakamoto, Renato Amaral, Pietro Sampaio Baruselli, Takako Fujii, Leonardo José Richtzenhain
Format: Article
Language:Portuguese
Subjects:
Bubalus bubalis
Búfalos
ELISA
Rinotraquite infecciosa bovina
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Summary:The performance of a commercial ELISA (Bovine Rhinotracheitis virus antibody test Kit, Herdcheck®, IDEXX Laboratories Inc., USA) designed to detect the presence of antibody to the Bovine Herpesvirus type 1 (BHV-1) in bovine serum, was evaluated in samples of buffaloes (Bubalus bubalis) serum. Sera of 133 buffaloes without IBR vaccination were performed both by ELISA and standard serum neutralization technique. Sensitivity and specificity of ELISA were 97.14% and 46.03%, respectively. The estimated concordance between two serologic diagnostic methods by kappa index was 0.44 IC (0.29-0.59) using 95% confidence level. O desempenho de um ELISA comercial (Bovine Rhinotracheitis virus antibody test Kit, Herdcheck®, IDEXX Laboratories Inc., USA) para a identificação de anticorpos anti-herpesvírus bovino tipo 1 (BHV-1), em soro bovino, foi avaliado em 133 amostras de soro de búfalos (Bubalus bubalis), sem histórico de vacinação para IBR, da Região do Vale do Ribeira, Estado de São Paulo. Tomando-se a técnica de soroneutralização como referência, o ELISA indireto apresentou sensibilidade e especificidade relativa de 97,14 e 46,03%, respectivamente. A concordância estimada pelo índice kappa, entre os dois métodos, foi de 0,44 IC (0,29-0,59), utilizando um intervalo de confiança de 95%.
ISSN:1413-9596
1678-4456