Cell wall degradation of Bouteloua repens in vitro by pure cultures of R. flavefaciens and F. succinogenes isolate from cattle grazing tropical lowland pastures in Colombia

The capacity to degrade the cellular wall of Bouteloua repens of 12 isolates of Ruminococcus flavefaciens and 17 isolates of Fibrobacter succinogenes was determined. A procedure was developed to standardize both, the inoculum and the substrate concentration for in vitro incubations of pure culture i...

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Bibliographic Details
Published in:Ciencia y Tecnología Agropecuaria 2013-12
Main Authors: Ossa, Faisury, Arcos, Martha, Díaz, Tito E, Pittroff, Wolfgang
Format: Article
Language:Spanish
Online Access:Get full text
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Summary:The capacity to degrade the cellular wall of Bouteloua repens of 12 isolates of Ruminococcus flavefaciens and 17 isolates of Fibrobacter succinogenes was determined. A procedure was developed to standardize both, the inoculum and the substrate concentration for in vitro incubations of pure culture isolates.The ruminal bacteria were isolated from either Bos indicus cattle from the Alto Magdalena region of Colombia, grazing Bouteloua repens, and Sanmartinero cows (a Colombian criollo breed) from the  eastern lowlands (Llanos Orientales) of Colombia, grazing Brachiara spp. The isolates identity was confirmed using molecular methods. All isolates of F. succinogenes showed a high capacity to degrade cell wall preparations of Bouteloua repens, than those of R. flavefaciens (P
ISSN:2500-5308
0122-8706