Characterization of fowl adenoviruses isolated from field outbreaks of hydropericardium syndrome in Punjab

Fowl adenovirus(FAV) isolaes (LHPSV - 2, 4, 6 AND 7) obtained from field outbreaks of hydropericardium syndrome (HPS) were characterized as serotype- 4 of FAV using virus neutralization test. The pathogenicity of isolate LHPSV- 7 was studied in experimental birds. Clinical disease appeared on 3 days...

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Bibliographic Details
Published in:Indian journal of animal sciences 2004-05, Vol.74 (5)
Main Authors: Singh, A, Kumari, A, Maiti, N.K, Oberoi, M.S. (Punjab Agricultural University, Ludhiana (India))
Format: Article
Language:English
Subjects:
broiler chickens
elisa
immunological techniques
pathogenicity
symptoms
virology
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Summary:Fowl adenovirus(FAV) isolaes (LHPSV - 2, 4, 6 AND 7) obtained from field outbreaks of hydropericardium syndrome (HPS) were characterized as serotype- 4 of FAV using virus neutralization test. The pathogenicity of isolate LHPSV- 7 was studied in experimental birds. Clinical disease appeared on 3 days post inoculation (DPI) of HPS isolate (LHPSV-7) in 3- week- old disease free broiler chicks. In experimental birds 16% mortality was observed. The severity of disease was seen between 5 and 7 DPI. The dead and sacrificed birds showed characteristic hytdropericardium with transparent, amber coloured fluid in the pericardial cavity. Characeristic pathological changes in liver included, necrotic hepatitis and intrauclear inclusion bodies in hepatocytes. The inclusions were also observed in kidneys, heart, spleen and pancreas at variable stages of infection. Microscopically, depletion of lymphoid cells was observed in bursa, thymus, spleen and caecal tonsils. FAV antigen was detected at 7, 13 and 21 DPI in plasma by antigen capture ELISA. Antibodies to FAV were detedted in serum by AGPT and indeirect ELISA from 7 DPE on wards. A high titre (GMT 1:64) was seen first on 3 DPI followed by a decrease in antibody titre, which again increased by 21 DPI (GMT1:53.81).
ISSN:0367-8318